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Author Notes:

Address correspondence to Monica Serra, PhD, San Antonio GRECC, South Texas VA and the Division of Geriatrics, Gerontology & Palliative Medicine and the Sam & Ann Barshop Institute for Longevity & Aging Studies, UT Health San Antonio, 7400 Merton Minter (182-GRECC), San Antonio, TX 78229, serram@uthscsa.edu

Acknowledgements Our appreciation is extended to the volunteers who participated in this study.

Conflict of Interest None.


Research Funding:

This study was supported by funds from a CDA-2 Number IK2 RX-000944 and a Senior Research Career Scientist Award from the United States (U.S.) Department of Veterans Affairs Rehabilitation R&D (Rehab RD) Service, the San Antonio, Birmingham/Atlanta, and Baltimore VA GRECCs, and Atlanta VA CVNR (Grant Number C2358-C), and NIH R01-AG030075.


  • Science & Technology
  • Life Sciences & Biomedicine
  • Neurosciences
  • Peripheral Vascular Disease
  • Neurosciences & Neurology
  • Cardiovascular System & Cardiology
  • Stroke
  • metabolomics
  • exercise
  • aerobic fitness
  • RISK

Metabolomics of Aerobic Exercise in Chronic Stroke Survivors: A Pilot Study


Journal Title:

Journal of Stroke and Cerebrovascular Diseases


Volume 28, Number 12


, Pages 104453-104453

Type of Work:

Article | Post-print: After Peer Review


Background: Understanding the metabolic response to exercise may aid in optimizing stroke management. Therefore, the purpose of this pilot study was to evaluate plasma metabolomic profiles in chronic stroke survivors following aerobic exercise training. Methods: Participants (age: 62 ± 1 years, body mass index: 31 ± 1 kg/m2, mean ± standard error of the mean) were randomized to 6 months of treadmill exercise (N = 17) or whole-body stretching (N = 8) with preintervention and postintervention measurement of aerobic capacity (VO2peak). Linear models for microarray data expression analysis was performed to determine metabolic changes over time, and Mummichog was used for pathway enrichment analysis following analysis of plasma samples by high-performance liquid chromatography coupled to ultrahigh resolution mass spectrometry. Results: VO2peak change was greater following exercise than stretching (18.9% versus −.2%; P <. 01). Pathway enrichment analysis of differentially expressed metabolites results showed significant enrichment in 4 pathways following treadmill exercise, 3 of which (heparan-, chondroitin-, keratan-sulfate degradation) involved connective tissue metabolism and the fourth involve lipid signaling (linoleate metabolism). More pathways were altered in pre and post comparisons of stretching, including branched-chain amino acid, tryptophan, tyrosine, and urea cycle, which could indicate loss of lean body mass. Conclusions: These preliminary data show different metabolic changes due to treadmill training and stretching in chronic stroke survivors and suggest that in addition to improved aerobic capacity, weight-bearing activity, like walking, could protect against loss of lean body mass. Future studies are needed to examine the relationship between changes in metabolomic profiles to reductions in cardiometabolic risk after treadmill rehabilitation.

Copyright information:

© 2019

This is an Open Access work distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/).
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