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Author Notes:

Correspondence: Jessica Alvarez 101 Woodruff Cr NE-1028 WMRB Atlanta, GA 30322 Ph: (404) 727-1549 Fax: (404) 727-1300 jessica.alvarez@emory.edu

We thank the anonymous reviewers for their insightful contributions to improving the manuscript.

The authors have nothing to disclose.


Research Funding:

Information upon which this work is based is from the Emory/Georgia Tech Predictive Health Participant Database, and is supported by the National Center for Advancing Translational Sciences of the National Institutes of Health (UL1 TR000454).

Other sources of support for this study include grants from the National Institutes of Health (K23 AR054334 (VT), T32 DK007298-32S1 (JAA), and K24 RR023356 (TRZ)).


  • Science & Technology
  • Life Sciences & Biomedicine
  • Endocrinology & Metabolism

Vitamin D status is independently associated with plasma glutathione and cysteine thiol/disulphide redox status in adults


Journal Title:

Best Practice and Research: Clinical Endocrinology and Metabolism


Volume 81, Number 3


, Pages 458-466

Type of Work:

Article | Post-print: After Peer Review


Objective Redox status and inflammation are important in the pathophysiology of numerous chronic diseases. Epidemiological studies have linked vitamin D status to a number of chronic diseases. We aimed to examine the relationships between serum 25-hydroxyvitamin D [25(OH)D] and circulating thiol/disulphide redox status and biomarkers of inflammation. Design This was a cross-sectional study of N = 693 adults (449 females, 244 males) in an apparently healthy, working cohort in Atlanta, GA. Plasma glutathione (GSH), cysteine (Cys) and their associated disulphides were determined with high-performance liquid chromatography, and their redox potentials (E h GSSG and Eh CySS) were calculated using the Nernst equation. Serum inflammatory markers included interleukin-6 (IL-6), interleukin-8 (IL-8) and tumour necrosis factor-α, assayed on a multiplex platform, and C-reactive protein (CRP), assayed commercially. Relationships were assessed with multiple linear regression analyses. Results Serum 25(OH)D was positively associated with plasma GSH (β ± SE: 0·002 ± 0·0004) and negatively associated with plasma Eh GSSG (β ± SE: -0·06 ± 0·01) and Cys (β ± SE: -0·01 ± 0·003) (P < 0·001 for all); statistical significance remained after adjusting for age, gender, race, percentage body fat and traditional cardiovascular risk factors (P = 0·01-0·02). The inverse relationship between serum 25(OH)D and CRP was confounded by percentage body fat, and full adjustment for covariates attenuated serum 25(OH)D relationships with other inflammatory markers to nonstatistical significance. Conclusions Serum 25(OH)D concentrations were independently associated with major plasma thiol/disulphide redox systems, suggesting that vitamin D status may be involved in redox-mediated pathophysiology.

Copyright information:

© 2014 John Wiley & Sons Ltd.

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