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Author Notes:

Sheila T. Angeles-Han, Email: sheila.angeles-han@cchmc.org.

All authors were involved in drafting the article or revising it critically for important intellectual content, and all authors approved the final version to be submitted for publication.

STA-H is accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.

STA-H, SP, and GNH contributed to the study conception and design.

STA-H, KJ, PP, KAR-S, and SY did the acquisition of the data.

STA-H, MA, DD, NF, GNH, and ST took part in the analysis and interpretation of the data.

The authors thank Theresa Hennard and Najima Mwase for their administrative assistance.

Dr. Steven Yeh is a consultant for Santen and Clearside Biomedical not related to this study.

Dr. Prahalad has served on an advisory committee for Novartis Pharmaceuticals not related to this study.

The other authors declare that they have no competing interests.


Research Funding:

Dr. Angeles-Han was supported by Award Number K23EY021760 from the National Eye Institute, the Rheumatology Research Foundation, and the Cincinnati Children’s Hospital Medical Center Research Innovation and Pilot fund.

These grants supported the design of the study and collection, analysis, interpretation of the data, and in writing the manuscript.

Dr. Prahalad is supported, in part, by a grant from the Marcus Foundation Inc., Atlanta.

Dr. Holland is supported by the Maggi Kelly Vision Fund (UCLA Stein Eye Institute) and the Fund for Children with Uveitis (UCLA Stein Eye Institute).


  • Biomarkers
  • Juvenile idiopathic arthritis
  • Uveitis

Discovery of tear biomarkers in children with chronic non-infectious anterior uveitis: a pilot study

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Journal Title:

Journal of Ophthalmic Inflammation and Infection


Volume 8, Number 1


, Pages 17-17

Type of Work:

Article | Final Publisher PDF


Background: Biomarkers in easily obtained specimens that accurately predict uveitis in children with juvenile idiopathic arthritis (JIA) are needed. Aqueous humor has been studied for biomarkers, but is not routinely available. We evaluated tears from children with chronic anterior uveitis (CAU) for biomarkers reported in aqueous humor. In this pilot study, we used Schirmer strips to collect tears from seven children (nine eyes); three children had JIA- associated uveitis (JIA-U) and four had idiopathic disease (I-CAU). Liquid chromatography-tandem mass spectrometry was used to identify and quantify tear proteins. The Mann-Whitney U test identified differential tear protein expression between children with JIA-U and those with I-CAU. Results: S100A9, LAP3, TTR, MIF, sCD14, S100A8, and SAA1 were detected in tears of all children; the same cytokines have been reported in aqueous humor of children with JIA-U. Tears from children with JIA-U had higher expression of proteins associated with inflammatory arthritis (SEMA3G, TIMP1, HEXB, ERN1, and SAA1) than tears from those with I-CAU. In addition, we found higher expression of sCD14, S100A8, and SAA1, but lower expression of S100A9, LAP3, TTR, and MIF, in tears from children with JIA-U compared to tears from those with I-CAU. Conclusions: Tears contain similar cytokine profiles to aqueous humor in children with CAU and may be a clinically useful source of disease biomarkers. Tears from children with JIA-U also contain cytokines associated with inflammatory arthritis; furthermore, differential expression of other tear proteins as well may provide clues to intrinsic differences between JIA-U and I-CAU, despite their similar clinical phenotypes.

Copyright information:

© 2018, The Author(s).

This is an Open Access work distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/).
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