About this item:

265 Views | 151 Downloads

Author Notes:

Correspondence to: Iain T. Shepherd, Department of Biology, Emory University, Atlanta, GA. E‐mail: ishephe@emory.edu

We like to thank members of the shepherd lab for technical support.

We’d also like to thank Dr. Andreas Fritz and Dr. Steve L’Hernault for comments on the manuscript.

Subject:

Research Funding:

This work was supported by a URC grant from Emory College and a T32 training grant.

Keywords:

  • Enteric nervous system
  • phox2b
  • Kaede
  • in vivo
  • med24

In vivo visualization of the development of the enteric nervous system using a Tg(−8.3bphox2b:Kaede) transgenic zebrafish

Tools:

Share

Journal Title:

genesis

Volume:

Volume 52, Number 12

Publisher:

, Pages 985-990

Type of Work:

Article | Post-print: After Peer Review

Abstract:

The phox2b gene encodes a transcription factor that is expressed in the developing enteric nervous system (ENS). An enhancer element has been identified in the zebrafish phox2b locus that can drive tissue specific expression of reporter genes in enteric neuron precursor cells. We have generated a transgenic zebrafish line in which the Kaede fluorescent protein is under the control of this phox2b enhancer. This line has stable expression of the Kaede protein in enteric neuron precursor cells over 3 generations. To demonstrate the utility of this line we compared the migration and division rates of enteric neuron precursor cells in wild type and the zebrafish ENS mutant lessen.

Copyright information:

© 1999-2018 John Wiley & Sons, Inc. All rights reserved

Export to EndNote
Site Statistics

Copyright © 2016 Emory University - All Rights Reserved
540 Asbury Circle, Atlanta, GA 30322-2870
(404) 727-6861
Privacy Policy | Terms & Conditions

v2.2.8-dev

Contact Us Recent and Popular Items
Download now