Detection of Immunoglobulin G Antibodies to Taenia solium Cysticercosis Antigen Glutathione-S-Transferase-rT24H in Malian Children Using Multiplex Bead Assay

• Type of Material: Article
• Authors: Delynn M. Moss, Centers for Disease Control & Prevention; Sukwan Handali, Centers for Disease Control & Prevention; Anna N. Chard, Emory University; Victoria Trinies, Emory University; Steven Bullard, Centers for Disease Control & Prevention; Ryan E. Wiegand, Centers for Disease Control & Prevention; Seyou Doumbia, University of Sciences Techniques & Technology; Matthew Freeman, Emory University; Patrick Lammie, Emory University
• Language: English
• Date: 2018-01-01
• Publisher: American Society of Tropical Medicine and Hygiene
• Publication Version: Final Published Version
• Copyright Statement: Copyright © 2018 by The American Society of Tropical Medicine and Hygiene.
• Final Published Version (URL): http://dx.doi.org/10.4269/ajtmh.17-0310
• Title of Journal or Parent Work: American Journal of Tropical Medicine and Hygiene
• ISSN: 0002-9637
• Volume: 98
• Issue: 5
• Start Page: 1408
• End Page: 1412
• Keywords: Tropical Medicine; NEUROCYSTICERCOSIS; Science & Technology; Public, Environmental & Occupational Health; Life Sciences & Biomedicine; DIAGNOSIS; IMPACT
• Research Categories: Health Sciences, Public Health; Biology, Microbiology

Abstract

Blood samples from 805 students attending 42 elementary schools in Mopti, Sikasso, and Koulikoro regions, and Bamako district in Mali participated in a school water, sanitation, and hygiene intervention. Immunoglobulin (Ig) G responses to several antigens/pathogens were assessed by a multiplex bead assay (MBA), and the recombinant Taenia solium T24H antigen was included. Of all students tested, 8.0% were positive to rT24H, but in some schools 25–30%. A cluster of 12 widespread school locations showed not only a relative risk of 3.23 for T. solium exposure and significantly higher IgG responses (P < 0.001) but also significantly lower elevation (P = 0.04) (m, above sea level) compared with schools outside the cluster. All schools at elevations < 425 m showed significantly higher IgG responses (P = 0.017) than schools at elevations 3 425 m. The MBA is an excellent serological platform that provides cost-effective opportunities to expand testing in serosurveys.