Impact of pre-existing dengue immunity on human antibody and memory B cell responses to Zika

• Type of Material: Article
• Authors: Paulina Andrade, University of California Berkeley; Ciara Gimblet-Ochieng, University of North Carolina; Faraz Modirian, University of California Berkeley; Matthew Collins, Emory University; Maritza Cardenas, University of California Berkeley; Leah C. Katzelnick, University of California Berkeley; Magelda Montoya, University of California Berkeley; Daniela Michlmayr, University of California Berkeley; Guillermina Kuan, Ministry of Health; Angel Balmaseda, Sustainable Sciences Institute; Josefina Coloma, University of California Berkeley; Aravinda M. de Silva, University of North Carolina; Eva Harris, University of California Berkeley
• Language: English
• Date: 2019-02-26
• Publisher: Nature Research (part of Springer Nature): Fully open access journals
• Publication Version: Final Published Version
• Copyright Statement: © 2019, The Author(s).
• License: Creative Commons Attribution 4.0 International
• Final Published Version (URL): http://dx.doi.org/10.1038/s41467-019-08845-3
• Title of Journal or Parent Work: Nature Communications
• ISSN: 2041-1723
• Volume: 10
• Issue: 1
• Start Page: 938
• End Page: 938
• Grant/Funding Information: The Pediatric Dengue Cohort Study was also supported by the Pediatric Dengue Vaccine Initiative grant VE-1 (E.H.) from the Bill and Melinda Gates Foundation and by the FIRST grant (E.H. and J.C.) from the Bill and Melinda Gates Foundation and the Instituto Carlos Slim de la Salud.; This work was supported by grants P01AI106695 (EH), U19AI118610 (EH), and R01AI099631 (AB) from the National Institute of Allergy and Infectious Diseases (NIH).
• Keywords: VACCINATION; YELLOW-FEVER VIRUS; Science & Technology; Multidisciplinary Sciences; NEUTRALIZING ANTIBODIES; CHIKUNGUNYA; CROSS-REACTIVITY; REPLICATION; Science & Technology - Other Topics; DISEASE SEVERITY; DEPENDENT ENHANCEMENT; MULTICOLOR FLUOROSPOT; INFECTION
• Research Categories: Biology, Microbiology; Biology, Virology; Health Sciences, Immunology

Abstract

Little is known about enduring memory B cell (MBC) responses to Zika virus (ZIKV) and their relationship with circulating antibodies. Here we comprehensively assess MBC frequency and specificity alongside serum binding and neutralizing antibody responses to ZIKV ~2 weeks and ~8 months postinfection in 31 pediatric subjects with 0, 1 or >1 prior infections with the related dengue virus (DENV). ZIKV infection elicits a robust type-specific MBC response, and the majority of late convalescent anti-ZIKV serum neutralizing activity is attributable to ZIKV-specific antibodies. The number of prior DENV infections does not influence type-specific or cross-reactive MBC responses, although ZIKV has the highest cross-reactivity with DENV3. DENV cross-reactive MBCs expanded by ZIKV infection decline in number and proportion by late convalescence. Finally, ZIKV induces greater cross-reactivity in the MBC pool than in serum antibodies. Our data suggest immunity to DENV only modestly shapes breadth and magnitude of enduring ZIKV antibody responses.