Publication

Enhanced Pro-apoptotic Effects of Fe(II)-Modified IVIG on Human Neutrophils

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Last modified
  • 05/15/2025
Type of Material
Authors
    Stefanie Graeter, University of BernChristoph Schneider, University of BernDanielle Verschoor, University of BernSandro von Daeniken, University of BernFrank Seibold, Hochhaus LindenhofspitalNikhil Yawalkar, University Hospital, Bern, SwitzerlandPeter Villiger, University Hospital, Bern, SwitzerlandJordan D. Dimitrov, Sorbonne UniversiteDavid Smith, Emory UniversityRichard Cummings, Emory UniversityHans-Uwe Simon, University of BernTchavdar Vassilev, Bulgarian Academy of SciencesStephan von Gunten, University of Bern
Language
  • English
Date
  • 2020-05-19
Publisher
  • Frontiers Media S.A.
Publication Version
Copyright Statement
  • © 2020 Graeter, Schneider, Verschoor, von Däniken, Seibold, Yawalkar, Villiger, Dimitrov, Smith, Cummings, Simon, Vassilev and von Gunten.
License
Final Published Version (URL)
Title of Journal or Parent Work
Volume
  • 11
Start Page
  • 973
End Page
  • 973
Grant/Funding Information
  • This work was supported in part by the Bulgarian—Swiss Research Program (BSRP) No. IZEBZO_142967, and the Swiss National Science Foundation grants 310030_162552 and 310030_184757 to SGu, and NIH/NIGMS grants P41GM103694 and GM098791 to RC and H-US received support from the Swiss National Science Foundation (grants Nos. 310030-166473 and 310030_184816) and the European Union's Horizon 2020 research and innovation program (Marie Sklodowska-Curie grant No. 642295; MEL-PLEX).
Supplemental Material (URL)
Abstract
  • Mild modification of intravenous immunoglobulin (IVIG) has been reported to result in enhanced polyspecificity and leveraged therapeutic effects in animal models of inflammation. Here, we observed that IVIG modification by ferrous ions, heme or low pH exposure, shifted the repertoires of specificities in different directions. Ferrous ions exposed Fe(II)-IVIG, but not heme or low pH exposed IVIG, showed increased pro-apoptotic effects on neutrophil granulocytes that relied on a FAS-dependent mechanism. These effects were also observed in human neutrophils primed by inflammatory mediators or rheumatoid arthritis joint fluid in vitro, or patient neutrophils ex vivo from acute Crohn's disease. These observations indicate that IVIG-mediated effects on cells can be enhanced by IVIG modification, yet specific modification conditions may be required to target specific molecular pathways and eventually to enhance the therapeutic potential.
Author Notes
Keywords
Research Categories
  • Health Sciences, Immunology
  • Chemistry, Biochemistry
  • Biology, Cell
  • Health Sciences, Rehabilitation and Therapy

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