Hypertonic saline reduces lipopolysaccharide-induced mouse brain edema through inhibiting aquaporin 4 expression

• Type of Material: Article
• Authors: C Cao, Central South University; X Yu, Central South University; Z Liao, Central South University; N Zhu, Central South University; H Huo, Central South University; M Wang, Central South University; G Ji, Central South University; Hua She, Emory University; Z Luo, Central South University; S Yue, Central South University
• Language: English
• Date: 2012-10-04
• Publisher: BioMed Central
• Publication Version: Final Published Version
• Copyright Statement: © 2012 Cao et al.; licensee BioMed Central Ltd.
• License: Creative Commons Attribution 2.0 Generic
• Final Published Version (URL): http://dx.doi.org/10.1186/cc11670
• Title of Journal or Parent Work: Critical Care Nursing Quarterly
• ISSN: 0887-9303
• Volume: 16
• Issue: 5
• Start Page: R186
• End Page: R186
• Grant/Funding Information: This study was supported by grants from the National Natural Science Foundation of China (No: 81070970), Specialized Research Fund for the Doctoral Program of Higher Education (No: 20090162110016), the Open-End Fund for the Valuable and Precision Instruments of Central South University and Research Fund of Development and Research Fund of Development and Reform Commission of Hunan Province (No:2010).
• Keywords: RAT-BRAIN; Critical Care Medicine; Life Sciences & Biomedicine; CRITICAL CARE MEDICINE; PLASMA-MEMBRANES; CEREBRAL EDEMA; hypertonic saline; INCREASED INTRACRANIAL-PRESSURE; brain edema; 20-PERCENT MANNITOL; BARRIER DISRUPTION; SEPTIC ENCEPHALOPATHY; Science & Technology; PROTEIN-KINASE-C; protein kinase C; PHORBOL-MYRISTATE ACETATE; interleukin 1 beta; General & Internal Medicine; aquaporin 4; EXPERIMENTAL INTRACEREBRAL HEMORRHAGE; lipopolysaccharide
• Research Categories: Biology, Physiology; Health Sciences, Pharmacology

Abstract

Introduction: Three percent sodium chloride (NaCl) treatment has been shown to reduce brain edema and inhibited brain aquaporin 4 (AQP4) expression in bacterial meningitis induced by Escherichia coli. Lipopolysaccharide (LPS) is the main pathogenic component of E. coli. We aimed to explore the effect of 3% NaCl in mouse brain edema induced by LPS, as well as to elucidate the potential mechanisms of action. Methods: Three percent NaCl was used to treat cerebral edema induced by LPS in mice in vivo. Brain water content, IL-1β, TNFα, immunoglobulin G (IgG), AQP4 mRNA and protein were measured in brain tissues. IL-1β, 3% NaCl and calphostin C (a specific inhibitor of protein kinase C) were used to treat the primary astrocytes in vitro. AQP4 mRNA and protein were measured in astrocytes. Differences in various groups were determined by one-way analysis of variance. Results: Three percent NaCl attenuated the increase of brain water content, IL-1β, TNFα, IgG, AQP4 mRNA and protein in brain tissues induced by LPS. Three percent NaCl inhibited the increase of AQP4 mRNA and protein in astrocytes induced by IL-1β in vitro. Calphostin C blocked the decrease of AQP4 mRNA and protein in astrocytes induced by 3% NaCl in vitro. Conclusions: Osmotherapy with 3% NaCl ameliorated LPS-induced cerebral edema in vivo. In addition to its osmotic force, 3% NaCl exerted anti-edema effects possibly through down-regulating the expression of proinflammatory cytokines (IL-1β and TNFα) and inhibiting the expression of AQP4 induced by proinflammatory cytokines. Three percent NaCl attenuated the expression of AQP4 through activation of protein kinase C in astrocytes.