In Vitro Assessment of the Immunological Significance of a Human Monoclonal Antibody Directed to the Influenza A Virus Nucleoprotein

• Type of Material: Article
• Authors: Rogier Bodewes, Erasmus MC; Martina M. Geelhoed-Mieras, Erasmus MC; Jens Wrammert, Emory University; Rafi Ahmed, Emory University; Patrick C. Wilson, University of Chicago; Ron A. M. Fouchier, Erasmus MC; Albert D. M. E. Osterhaus, Erasmus MC; Guus F. Rimmelzwaan, Erasmus MC
• Language: English
• Date: 2013-08-01
• Publisher: American Society for Microbiology
• Publication Version: Final Published Version
• Copyright Statement: © 2013, American Society for Microbiology.
• Final Published Version (URL): http://dx.doi.org/10.1128/CVI.00339-13
• Title of Journal or Parent Work: Clinical and Vaccine Immunology
• ISSN: 1556-6811
• Volume: 20
• Issue: 8
• Start Page: 1333
• End Page: 1337
• Keywords: HETEROSUBTYPIC IMMUNITY; EPITOPE; CYTOTOXIC T-LYMPHOCYTE; VACCINATION; Life Sciences & Biomedicine; CELLS; Infectious Diseases; H1N1; INFECTION; INDUCTION; Microbiology; Science & Technology; RESPONSES; Immunology
• Research Categories: Biology, Virology; Health Sciences, Immunology

Abstract

Influenza A viruses cause annual epidemics and occasionally pandemics. Antibodies directed to the conserved viral nucleoprotein (NP) may play a role in immunity against various influenza A virus subtypes. Here, we assessed the immunological significance of a human monoclonal antibody directed to NP in vitro. This antibody bound to virus-infected cells but did not display virus-neutralizing activity, complement-dependent cell cytotoxicity, or opsonization of viral antigen for improved antigen presentation to CD8 + T cells by dendritic cells.