Vitamin D status is independently associated with plasma glutathione and cysteine thiol/disulphide redox status in adults

• Type of Material: Article
• Authors: Jessica A. Alvarez, Emory University; Ritam Chowdhury, Emory University; Dean P Jones, Emory University; Greg Martin, Emory University; Kenneth L Brigham, Emory University; Jose N. Binongo, Emory University; Thomas R Ziegler, Emory University; Vin Tangpricha, Emory University
• Language: English
• Date: 2014-09-01
• Publisher: Elsevier: 12 months
• Publication Version: Author Accepted Manuscript (After Peer Review)
• Copyright Statement: © 2014 John Wiley & Sons Ltd.
• Final Published Version (URL): http://dx.doi.org/10.1111/cen.12449
• Title of Journal or Parent Work: Best Practice and Research: Clinical Endocrinology and Metabolism
• ISSN: 1521-690X
• Volume: 81
• Issue: 3
• Start Page: 458
• End Page: 466
• Grant/Funding Information: Information upon which this work is based is from the Emory/Georgia Tech Predictive Health Participant Database, and is supported by the National Center for Advancing Translational Sciences of the National Institutes of Health (UL1 TR000454).; Other sources of support for this study include grants from the National Institutes of Health (K23 AR054334 (VT), T32 DK007298-32S1 (JAA), and K24 RR023356 (TRZ)).
• Keywords: PHYSICAL-ACTIVITY; MARKERS; SERUM 25-HYDROXYVITAMIN D; OXIDATIVE STRESS; Endocrinology & Metabolism; BIOMARKERS; SUPPLEMENTATION; Science & Technology; HEALTH; CHOLECALCIFEROL; ENDOTHELIAL FUNCTION; Life Sciences & Biomedicine; INFLAMMATION
• Research Categories: Health Sciences, Epidemiology; Health Sciences, Nutrition

Abstract

Objective Redox status and inflammation are important in the pathophysiology of numerous chronic diseases. Epidemiological studies have linked vitamin D status to a number of chronic diseases. We aimed to examine the relationships between serum 25-hydroxyvitamin D [25(OH)D] and circulating thiol/disulphide redox status and biomarkers of inflammation. Design This was a cross-sectional study of N = 693 adults (449 females, 244 males) in an apparently healthy, working cohort in Atlanta, GA. Plasma glutathione (GSH), cysteine (Cys) and their associated disulphides were determined with high-performance liquid chromatography, and their redox potentials (E h GSSG and Eh CySS) were calculated using the Nernst equation. Serum inflammatory markers included interleukin-6 (IL-6), interleukin-8 (IL-8) and tumour necrosis factor-α, assayed on a multiplex platform, and C-reactive protein (CRP), assayed commercially. Relationships were assessed with multiple linear regression analyses. Results Serum 25(OH)D was positively associated with plasma GSH (β ± SE: 0·002 ± 0·0004) and negatively associated with plasma Eh GSSG (β ± SE: -0·06 ± 0·01) and Cys (β ± SE: -0·01 ± 0·003) (P < 0·001 for all); statistical significance remained after adjusting for age, gender, race, percentage body fat and traditional cardiovascular risk factors (P = 0·01-0·02). The inverse relationship between serum 25(OH)D and CRP was confounded by percentage body fat, and full adjustment for covariates attenuated serum 25(OH)D relationships with other inflammatory markers to nonstatistical significance. Conclusions Serum 25(OH)D concentrations were independently associated with major plasma thiol/disulphide redox systems, suggesting that vitamin D status may be involved in redox-mediated pathophysiology.