Cytomegalovirus viral and antibody correlates in young children

• Type of Material: Article
• Authors: Sheila C Dollard, Centers for Disease Control and Prevention; Harry Keyserling, Emory University; Kay Radford, Centers for Disease Control and Prevention; Minal M Amin, Centers for Disease Control and Prevention; Jennifer Stowell, Centers for Disease Control and Prevention; Jörn Winter, Centers for Disease Control and Prevention; D Scott Schmid, Centers for Disease Control and Prevention; Michael J Cannon, Centers for Disease Control and Prevention; Terri B Hyde, Centers for Disease Control and Prevention
• Language: English
• Date: 2014-11-03
• Publisher: BioMed Central
• Publication Version: Final Published Version
• Copyright Statement: © Dollard et al.; licensee BioMed Central Ltd. 2014
• License: Creative Commons Attribution 2.0 Generic
• Final Published Version (URL): http://dx.doi.org/10.1186/1756-0500-7-776
• Title of Journal or Parent Work: BMC Research Notes
• ISSN: 1756-0500
• Volume: 7
• Issue: 1
• Start Page: 776
• End Page: 776
• Grant/Funding Information: Funding was provided by the Centers for Disease Control and Prevention.
• Keywords: Virus Shedding; Infant; Child Day Care Centers; Male; Humans; Antibody Affinity; Adult; Viral Load; Immunoglobulin G; Cytomegalovirus; Cytomegalovirus Infections; Pregnancy; Female; Antibodies, Viral; Child, Preschool; Child

Abstract

Background Young, healthy children shedding cytomegalovirus (CMV) in urine and saliva appear to be the leading source of CMV in primary infection of pregnant women. Findings We screened 48 children 6 months – 5 years old for CMV IgG and measured levels of CMV IgG, IgM and IgG avidity antibodies, frequency of CMV shedding, and viral loads in blood, urine, and saliva. Thirteen of the 48 children (27%) were CMV IgG positive, among whom 3 were also CMV IgM positive with evidence of recent primary infection. Nine of the 13 seropositive children (69%) were shedding 102-105 copies/ml of CMV DNA in one or more bodily fluid. Among seropositive children, low IgG antibody titer (1:20–1:80) was associated with the absence of shedding (p = 0.014), and enrollment in daycare was associated with the presence of CMV shedding (p = 0.037). Conclusions CMV antibody profiles correlated with CMV shedding. The presence of CMV IgM more often represents primary infection in children than in adults. Correlating antibodies with primary infection and viral shedding in healthy children adds to the understanding of CMV infection in children that can inform the prevention of CMV transmission to pregnant women.