Mitochondrial metabolomics using high-resolution fourier-transform mass spectrometry

• Type of Material: Article
• Authors: Young-Mi Go, Emory University; Karan Uppal, Emory University; James R. Roede, Emory University; Dean P Jones, Emory University; ViLinh Tran, Emory University; Douglas Walker, Emory University; Lauriane Dury, Université Lyon 1; Frederick Strobel, Emory University; Helene Baubichon-Cortay, Université Lyon 1; Kurt D. Pennell, Tufts University
• Language: English
• Date: 2014-01-01
• Publisher: Humana Press
• Publication Version: Author Accepted Manuscript (After Peer Review)
• Copyright Statement: © Springer Science+Business Media New York 2014.
• Final Published Version (URL): http://dx.doi.org/10.1007/978-1-4939-1258-2_4
• Title of Journal or Parent Work: Methods in Molecular Biology
• ISSN: 1064-3745
• Volume: 1198
• Start Page: 43
• End Page: 73
• Grant/Funding Information: This work was supported by NIH grants ES009047, HL113451, AG038746, ES016731; and NIAID Contract HHSN272201200031C.
• Keywords: Female; Mitochondria, Liver; Humans; Animals; Mice; Chromatography, Liquid; Fourier Analysis; Male; Mass Spectrometry; Metabolomics
• Research Categories: Health Sciences, Medicine and Surgery; Biology, Molecular; Chemistry, General

Abstract

High-resolution Fourier-transform mass spectrometry (FTMS) provides important advantages in studies of metabolism because more than half of common intermediary metabolites can be measured in 10 min with minimal pre-detector separation and without ion dissociation. This capability allows unprecedented opportunity to study complex metabolic systems, such as mitochondria. Analysis of mouse liver mitochondria using FTMS with liquid chromatography shows that sex and genotypic differences in mitochondrial metabolism can be readily distinguished. Additionally, differences in mitochondrial function are readily measured, and many of the mitochondria-related metabolites are also measurable in plasma. Thus, application of high-resolution mass spectrometry provides an approach for integrated studies of complex metabolic processes of mitochondrial function and dysfunction in disease.