Baseline host determinants of robust human HIV-1 vaccine-induced immune responses: A meta-analysis of 26 vaccine regimens

• Type of Material: Article
• Authors: Yunda Huang, Fred Hutchinson Cancer Center; Yuanyuan Zhang, Fred Hutchinson Cancer Center; Kelly E Seaton, Duke University; Stwphen De Rosa, Fred Hutchinson Cancer Center; Jack Heptinstall, Duke University; Lindsay N Carpp, Fred Hutchinson Cancer Center; April Kaur Randhawa, Fred Hutchinson Cancer Center; Lyle R McKinnon, University of Manitoba; Paul McLaren, University of Manitoba; Edna Viegas, Inst Nacl Saude; Glenda E Gray, University of Witwatersrand; Gavin Churchyard, Aurum Institute, Johannesburg; Susan P Buchbinder, San Francisco Department of Public Health; Srilatha Edupuganti, Emory University; Linda-Gail Bekker, University of Cape Town; Michael C Keefer, University of Rochester; Mina C Hosseinipour, University of North Carolina Project; Paul A Goepfert, University of Alabama Birmingham; Kristen W Cohen, Fred Hutchinson Cancer Center; Brian D Williamson, Fred Hutchinson Cancer Center; Juliana M McElrath, Fred Hutchinson Cancer Center; Georgia D Tomaras, Duke University; Juilee Thakar, University of Rochester; James J Kobie, University of Alabama Birmingham
• Language: English
• Date: 2022-10-01
• Publisher: ELSEVIER
• Publication Version: Final Published Version
• Copyright Statement: © 2022 The Author(s)
• License: Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International
• Final Published Version (URL): http://dx.doi.org/10.1016/j.ebiom.2022.104271
• Title of Journal or Parent Work: EBIOMEDICINE
• Volume: 84
• Start Page: 104271
• End Page: 104271
• Keywords: Research & Experimental Medicine; HETEROGENEITY; CELL RESPONSES; PLASMA; Vaccine response heterogeneity; CD4+ T cell; PROTECTION; CORRELATES ANALYSIS; General & Internal Medicine; Medicine, General & Internal; Science & Technology; SuperLearner; Antibody; ANTIBODIES; Life Sciences & Biomedicine; Medicine, Research & Experimental; Baseline characteristics; T-CELLS; DOUBLE-BLIND; Variable importance measurements; BINDING; EFFICACY TRIAL
• Research Categories: Health Sciences, Immunology; Health Sciences, Medicine and Surgery

Abstract

Background: The identification of baseline host determinants that associate with robust HIV-1 vaccine-induced immune responses could aid HIV-1 vaccine development. We aimed to assess both the collective and relative performance of baseline characteristics in classifying individual participants in nine different Phase 1-2 HIV-1 vaccine clinical trials (26 vaccine regimens, conducted in Africa and in the Americas) as High HIV-1 vaccine responders. Methods: This was a meta-analysis of individual participant data, with studies chosen based on participant-level (vs. study-level summary) data availability within the HIV-1 Vaccine Trials Network. We assessed the performance of 25 baseline characteristics (demographics, safety haematological measurements, vital signs, assay background measurements) and estimated the relative importance of each characteristic in classifying 831 participants as High (defined as within the top 25th percentile among positive responders or above the assay upper limit of quantification) versus Non-High responders. Immune response outcomes included HIV-1-specific serum IgG binding antibodies and Env-specific CD4+ T-cell responses assessed two weeks post-last dose, all measured at central HVTN laboratories. Three variable importance approaches based on SuperLearner ensemble machine learning were considered. Findings: Overall, 30.1%, 50.5%, 36.2%, and 13.9% of participants were categorized as High responders for gp120 IgG, gp140 IgG, gp41 IgG, and Env-specific CD4+ T-cell vaccine-induced responses, respectively. When including all baseline characteristics, moderate performance was achieved for the classification of High responder status for the binding antibody responses, with cross-validated areas under the ROC curve (CV-AUC) of 0.72 (95% CI: 0.68, 0.76) for gp120 IgG, 0.73 (0.69, 0.76) for gp140 IgG, and 0.67 (95% CI: 0.63, 0.72) for gp41 IgG. In contrast, the collection of all baseline characteristics yielded little improvement over chance for predicting High Env-specific CD4+ T-cell responses [CV-AUC: 0.53 (0.48, 0.58)]. While estimated variable importance patterns differed across the three approaches, female sex assigned at birth, lower height, and higher total white blood cell count emerged as significant predictors of High responder status across multiple immune response outcomes using Approach 1. Of these three baseline variables, total white blood cell count ranked highly across all three approaches for predicting vaccine-induced gp41 and gp140 High responder status. Interpretation: The identified features should be studied further in pursuit of intervention strategies to improve vaccine responses and may be adjusted for in analyses of immune response data to enhance statistical power. Funding: National Institute of Allergy and Infectious Diseases (UM1AI068635 to YH, UM1AI068614 to GDT, UM1AI068618 to MJM, and UM1 AI069511 to MCK), the Duke CFAR P30 AI064518 to GDT, and National Institute of Dental and Craniofacial Research (R01DE027245 to JJK). This work was also supported by the Bill and Melinda Gates Foundation. The content is solely the responsibility of the authors and does not necessarily represent the official views of any of the funding sources.