Measurement of Intestinal Permeability During Sepsis

• Type of Material: Article
• Authors: Takehiko Oami, Emory University; Craig Coopersmith, Emory University
• Language: English
• Date: 2021-01-01
• Publisher: HUMANA PRESS INC
• Publication Version: Author Accepted Manuscript (After Peer Review)
• Copyright Statement: © 2021 Springer Science+Business Media, LLC, part of Springer Nature
• Final Published Version (URL): http://dx.doi.org/10.1007/978-1-0716-1488-4_15
• Title of Journal or Parent Work: SEPSIS
• Volume: 2321
• Start Page: 169
• End Page: 175
• Grant/Funding Information: This work was supported by the National Institutes of Health under grants (GM072808, AA027396, GM104323, GM113228).
• Keywords: Medicine, Research & Experimental; Everted gut sac; Tight junction; CLAUDINS; Critical care; FD4; Gut; Research & Experimental Medicine; Biochemistry & Molecular Biology; GUT; Life Sciences & Biomedicine; Immunology; Sepsis; Biochemical Research Methods; Science & Technology; Permeability; Barrier; Intestine

Abstract

Gut barrier function has been hypothesized to play a critical role in the pathophysiology of sepsis. Measuring intestinal permeability allows for a determination of barrier dysfunction under conditions of health and disease. Fluorescence-conjugated dyes such as fluorescein isothiocyanate-4 kDa dextran (FD4) have been commonly used for evaluating hyperpermeability. Here we describe a common method to measure gut permeability in vivo, following gavage with different sized dyes. In addition, we describe an ex vivo everted gut sac model that allows for discrimination of permeability by segmental geographic location along the intestine.