Microarray-based resequencing of multiple Bacillus anthracis isolates

• Type of Material: Article
• Authors: Michael Zwick, Emory University; Farrell Mcafee, Naval Medical Research Center; David J Cutler, Johns Hopkins University School of Medicine; Timothy D Read, Emory University; Jacques Ravel, The Institute for Genomic Research; Gregory R Bowman, Naval Medical Research Center; Darrell R Galloway, Naval Medical Research Center; Alfred Mateczun, Naval Medical Research Center
• Language: English
• Date: 2005
• Publisher: BioMed Central
• Publication Version: Final Published Version
• Copyright Statement: © 2004 Zwick et al.; licensee BioMed Central Ltd.
• License: Creative Commons Attribution 2.0 Generic
• Final Published Version (URL): http://genomebiology.com/2004/6/1/R10
• Title of Journal or Parent Work: Genome Biology
• ISSN: 1465-6906
• Volume: 6
• Issue: 1
• Start Page: R10
• End Page: R10
• Grant/Funding Information: Funding from the Defense Threat Reduction Agency (DTRA) was used to support this study.
• Research Categories: Health Sciences, Epidemiology; Chemistry, Biochemistry; Biology, Genetics

Abstract

We used custom-designed resequencing arrays to generate 3.1 Mb of genomic sequence from a panel of 56 Bacillus anthracis strains. Sequence quality was shown to be very high by replication (discrepancy rate of 7.4 × 10-7) and by comparison to independently generated shotgun sequence (discrepancy rate < 2.5 × 10-6). Population genomics studies of microbial pathogens using rapid resequencing technologies such as resequencing arrays are critical for recognizing newly emerging or genetically engineered strains.