Angiogenin-mediated tRNA cleavage as a novel feature of stored red blood cells

• Type of Material: Article
• Authors: Wen-Hsuan Yang, Duke University; Jennifer F. Doss, Duke University; Katelyn A. Walzer, Duke University; Shannon M. McNulty, Duke University; Jianli Wu, Duke University; John Roback, Emory University; Jen-Tsan Chi, Duke University
• Language: English
• Date: 2019-05-01
• Publisher: Wiley
• Publication Version: Author Accepted Manuscript (After Peer Review)
• Copyright Statement: © 2019 John Wiley & Sons, Inc. All rights reserved.
• Final Published Version (URL): http://dx.doi.org/10.1111/bjh.15605
• Title of Journal or Parent Work: British Journal of Haematology
• Volume: 185
• Issue: 4
• Start Page: 760
• End Page: 764
• Grant/Funding Information: JD and KAW were supported by the Duke University Program in Genetics and Genomics. KAW was supported by the NSF Graduate Research Fellowship Program.; WHY was supported by the Taiwan Government Scholarship and the Duke Biochemistry Department.; This work was supported by the World Anti-Doping Agency (WADA) (13C31JC) and Partner for Clean Competition (to JTC) as well as R01 HL095479 and P01 HL 086773–06A1 (to JDR).
• Keywords: Hematology; Life Sciences & Biomedicine; angiogenin; tRNA fragment; Science & Technology; Storage; stored red blood cells; transfusion; miR-720
• Research Categories: Biology, Cell; Biology, Molecular; Biology, Genetics; Health Sciences, Pathology

Abstract

Red blood cell (RBC) transfusion is the most common therapeutic procedure in hospitalized patients. RBC units can be refrigerated for up to 42 days and undergo various biochemical and morphological changes during storage (storage lesions) (D’Alessandro, et al 2015). However, the effects of storage on the RBC transcriptome have not been well-studied. While once thought to lack nucleic acids, RBCs actually contain diverse and abundant RNA species (Chen, et al 2017). In addition, proteomic analyses of RBCs have identified the presence of Argonaute 2 (AGO2) (Bryk and Wisniewski 2017), supporting the regulatory function of miRNAs. Genomic analyses of the RBC transcriptome have provided insights into the heterogeneity and malaria resistance of sickle cell anaemia (Sangokoya, et al 2010, Walzer and Chi 2017) and several recent studies have begun to probe the effects of storage on the RBC transcriptome (Kannan and Atreya 2010, Sarachana, et al 2015).