Identification of genetic modifiers of age-at-onset for familial Parkinson's disease.

• Type of Material: Article
• Authors: Erin M. Hill-Burns, University of Alabama at Birmingham; Owen A. Ross, Mayo Clinic; William T. Wissemann, University of Alabama at Birmingham; Alexandra I. Soto-Ortolaza, Mayo Clinic; Sepideh Zareparsi, Oregon Health & Sciences University; Joanna Siuda, Medical University of Silesia; Timothy Lynch, University College Dublin; Zbigniew K. Wszolek, Mayo Clinic; Peter A. Silburn, Griffith University; George D. Mellick, Griffith University; Beate Ritz, University of California Los Angeles; Clemens R. Scherzer, Harvard University; Cyrus P. Zabetian, University of Washington; Stewart Factor, Emory University; Patrick J. Breheny, University of Iowa; Haydeh Payami, HudsonAlpha Institute for Biotechnology
• Language: English
• Date: 2016-09-01
• Publisher: Oxford University Press (OUP): Policy B - Oxford Open Option B
• Publication Version: Final Published Version
• Copyright Statement: © The Author 2016. Published by Oxford University Press.
• License: Creative Commons Attribution-NonCommercial 4.0 International
• Final Published Version (URL): http://dx.doi.org/10.1093/hmg/ddw206
• Title of Journal or Parent Work: Human Molecular Genetics
• ISSN: 0964-6906
• Volume: 25
• Issue: 17
• Start Page: 3849
• End Page: 3862
• Grant/Funding Information: Additional support was provided by National Institutes of Health [grant number P30AG08017]; a Merit Review Award from the Department of Veterans Affairs [grant number 1I01BX000531]; Office of Research & Development, Clinical Sciences Research & Development Service, Department of Veteran Affairs; and the Close to the Cure Foundation. Genome-wide array genotyping was conducted by the Center for Inherited Disease Research, which is funded by the National Institutes of Health [grant number HHSN268200782096C].; his work was supported by a grant from the National Institute of Neurological Disorders And Stroke [grant number R01NS036960].; Studies providing samples and data for replication were supported by National Institutes of Health [grant numbers U01NS082157, P50AG005134, R01ES010544, U54ES012078, R01NS078086 and P50NS72187]; a gift from Carl Edward Bolch, Jr. and Susan Bass Bolch; the American Parkinson's Disease Association; the Stowarzyszenie na Rzecz Rozwoju Neurologii Wieku Podeszlego grant; the Harvard NeuroDiscovery Center (HNDC); the Parkinson’s Disease Biomarkers Program (PDBP); the U.S. Department of Defense; and the M.E.M.O. Hoffman Foundation.
• Keywords: genes; genetics; genome-wide association study; age of onset; parkinson disease
• Research Categories: Health Sciences, Medicine and Surgery; Biology, Genetics

Abstract

Parkinson's disease (PD) is the most common cause of neurodegenerative movement disorder and the second most common cause of dementia. Genes are thought to have a stronger effect on age-at-onset of PD than on risk, yet there has been a phenomenal success in identifying risk loci but not age-at-onset modifiers. We conducted a genome-wide study for age-at-onset. We analysed familial and non-familial PD separately, per prior evidence for strong genetic effect on age-at-onset in familial PD. GWAS was conducted in 431 unrelated PD individuals with at least one affected relative (familial PD) and 1544 non-familial PD from the NeuroGenetics Research Consortium (NGRC); an additional 737 familial PD and 2363 non-familial PD were used for replication. In familial PD, two signals were detected and replicated robustly: one mapped to LHFPL2 on 5q14.1 (PNGRC = 3E-8, PReplication = 2E-5, PNGRC + Replication = 1E-11), the second mapped to TPM1 on 15q22.2 (PNGRC = 8E-9, PReplication = 2E-4, PNGRC + Replication = 9E-11). The variants that were associated with accelerated onset had low frequencies (<0.02). The LHFPL2 variant was associated with earlier onset by 12.33 [95% CI: 6.2; 18.45] years in NGRC, 8.03 [2.95; 13.11] years in replication, and 9.79 [5.88; 13.70] years in the combined data. The TPM1 variant was associated with earlier onset by 15.30 [8.10; 22.49] years in NGRC, 9.29 [1.79; 16.79] years in replication, and 12.42 [7.23; 17.61] years in the combined data. Neither LHFPL2 nor TPM1 was associated with age-at-onset in non-familial PD. LHFPL2 (function unknown) is overexpressed in brain tumours. TPM1 encodes a highly conserved protein that regulates muscle contraction, and is a tumour-suppressor gene.