Antibody Profiling by Proteome Microarray with Multiplex Isotype Detection Reveals Overlap between Human and Aotus nancymaae Controlled Malaria Infections

• Type of Material: Article
• Authors: Omid Taghavian, University of California Irvine; Aarti Jain, University of California Irvine; Chester J. Joyner, Emory University; Rie Nakajima, University of California Irvine; Algis Jasinskas, University of California Irvine; Li Liang, University of California Irvine; Rich Fong, Case Western Reserve University; Christopher King, Case Western Reserve University; Bryan Greenhouse, University of California San Francisco; Max Murphy, University of California San Francisco; Jason Bailey, Emory University; Mary Galinski, Emory University; John W. Barnwell, Centers for Disease Control and Prevention; Christopher V. Plowe, University of Maryland; D. Huw Davies, University of California Irvine; Philip L. Felgner, University of California Irvine
• Language: English
• Date: 2018-01-01
• Publisher: Wiley
• Publication Version: Author Accepted Manuscript (After Peer Review)
• Copyright Statement: © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
• Final Published Version (URL): http://dx.doi.org/10.1002/pmic.201700277
• Title of Journal or Parent Work: Proteomics
• ISSN: 1615-9853
• Volume: 18
• Issue: 2
• Start Page: 1700277
• End Page: 1700277
• Grant/Funding Information: Grace Bio Labs supplied free reagents for this research.; This project has been funded in part with Federal funds from the National Institute of Allergy and Infectious Diseases; National Institutes of Health, Department of Health and Human Services [Contract No. HHSN272201200031C] and the National Center for Research Resources [ORIP/OD P51OD011132].
• Keywords: Life Sciences & Biomedicine; Biochemical Research Methods; quantum dots; ASYMPTOMATIC PLASMODIUM-FALCIPARUM; HUMORAL IMMUNE-RESPONSES; VACCINE; IDENTIFICATION; Biochemistry & Molecular Biology; IMMUNOGLOBULIN-M; malaria; IGM; YELLOW-FEVER; multiplex; WESTERN THAILAND; antibody isotype; protein microarray; VALIDATION; LINKED IMMUNOSORBENT ASSAYS; Science & Technology
• Research Categories: Health Sciences, Public Health; Engineering, Biomedical; Health Sciences, Immunology

Abstract

The development of vaccines against malaria and serodiagnostic tests for detecting recent exposure requires tools for antigen discovery and suitable animal models. The protein microarray is a high-throughput, sample sparing technique, with applications in infectious disease research, clinical diagnostics, epidemiology, and vaccine development. We recently demonstrated Qdot-based indirect immunofluorescence together with portable optical imager ArrayCAM using single isotype detection could replicate data using the conventional laser confocal scanner system. We developed a multiplexing protocol for simultaneous detection of IgG, IgA, and IgM and compared samples from a controlled human malaria infection model with those from controlled malaria infections of Aotus nancymaae, a widely used non-human primate model of human malaria. IgG profiles showed the highest concordance in number of reactive antigens; thus, of the 139 antigens recognized by human IgG antibody, 111 were also recognized by Aotus monkeys. Interestingly, IgA profiles were largely non-overlapping. Finally, on the path toward wider deployment of the portable platform, we show excellent correlations between array data obtained in five independent laboratories around the United States using the multiplexing protocol (R2: 0.60–0.92). This study supports the use of this platform for wider deployment, particularly in endemic areas where such a tool will have the greatest impact on global human health.