Wnt Activation and Reduced Cell-Cell Contact Synergistically Induce Massive Expansion of Functional Human iPSC-Derived Cardiomyocytes

• Type of Material: Article
• Authors: Jan W Buikema, Stanford University; Soah Lee, Stanford University; William R Goodyer, Stanford University; Renee G Maas, Utrecht University; Orlando Chirikian, Stanford University; Guang Li, Stanford University; Yi Miao, Stanford University; Sharon L Paige, Stanford University; Daniel Lee, Stanford University; Haodi Wu, Stanford University; David T Paik, Stanford University; Siyeon Rhee, Stanford University; Lei Tian, Stanford University; Franciso X Galdos, Stanford University; Nazan Puluca, Stanford University; Benjamin Beyersdorf, Stanford University; James Hu, Stanford University; Aimee Beck, Stanford University; Sneha Venkamatran, Stanford University; Srilatha Swami, Stanford University; Paul Wijnker, University of Amsterdam; Maike Schuldt, University of Amsterdam; Larissa M Dorsch, University of Amsterdam; Alain van Mil, Utrecht University; Kristy Red-Horse, Stanford University; Joy Y Wu, Stanford University; Caroline Geisen, University of Bonn; Michael Hesse, University of Bonn; Vahid Serpooshan, Emory University; Stefan Jovinge, DeVos Cardiovascular Research Program of Spectrum Health and Van Andel Research Institute; Bernd K Fleischmann, University of Bonn; Pieter A Doevendans, Utrecht University; Jolanda van der Velden, University of Amsterdam; Christopher Garcia, Stanford University; Joseph C Wu, Stanford University; Joost PG Sluijter, Utrecht University; Sean M Wu, Stanford University
• Language: English
• Date: 2020-07-02
• Publisher: CELL PRESS
• Publication Version: Author Accepted Manuscript (After Peer Review)
• Copyright Statement: © 2020 Elsevier Inc.
• License: Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International
• Final Published Version (URL): http://dx.doi.org/10.1016/j.stem.2020.06.001
• Title of Journal or Parent Work: CELL STEM CELL
• Volume: 27
• Issue: 1
• Start Page: 50
• End Page: +
• Grant/Funding Information: The sequencing data was generated on an HiSeq purchased with funds from NIH under award number S10OD018220.; This work was supported by a UMC Utrecht Clinical Fellowship and Netherlands Heart Institute Fellowship (to J.B.); Stanford Child Health Research Institute Postdoctoral Fellowship and NIH NRSA Postdoctoral Fellowship 5F32HL142205 (to S.L.); NIH Pathway to Independence Award 1K99HL127295–01A1 (to V.S.); The Richard and Helen DeVos Foundation (to S.J.); Netherlands Heart Foundation (CVON-Dosis 2014–40), and Netherlands Organization for Sciences (NWO)-ZonMW (VICI 91818602) (to J.V.); R01 HL145676, R01 HL146690 and P01 HL141084 (to J.C.W); NIH (OD004411, HL099776, LM012179), and the Endowed Faculty Scholar Award of Lucile Packard Foundation for Children and Child Health Research Institute at Stanford (to S.M.W.).
• Keywords: STEM-CELLS; AKT KINASE; SIGNALING-PATHWAY; CANONICAL WNT; YAP; Science & Technology; DIFFERENTIATION; MECHANISMS; DISCOVERY; Cell & Tissue Engineering; Life Sciences & Biomedicine; PROLIFERATION; INHIBITION; Cell Biology

Abstract

Deriving a large number of hiPSC-cardiomyocytes would be beneficial for large-scale tissue engineering and drug screening applications. Buikema et al. show that GSK-3β inhibition combined with removal of cell-cell contact enables massive expansion of hiPSC-cardiomyocytes with comparable function to non-expanded cells.