New methodologies for the pathologic diagnosis of placental infections-immunohistochemistry, immunofluorescence, in situ nucleic acid hybridization and polymerase chain reaction: A review

• Type of Material: Article
• Authors: David A. Schwartz, Emory University; Eva Backé, Emory University; Wei Zhang, Emory University; Carlos Abramowsky, Emory University
• Language: English
• Date: 1994-01-01
• Publisher: Elsevier
• Publication Version: Final Published Version
• Copyright Statement: © 1994 Published by Elsevier Ltd.
• License: Creative Commons Attribution 4.0 International
• Final Published Version (URL): http://dx.doi.org/10.1016/S0143-4004(05)80350-9
• Title of Journal or Parent Work: Placenta
• Volume: 15
• Start Page: 251
• End Page: 261
• Grant/Funding Information: None declared
• Keywords: Immunohistochemistry techniques; Methodology; Immunofluorescence; Pathologic diagnosis; Nucleic acid hybridization; Pediatrics; Placenta infections
• Research Categories: Chemistry, Biochemistry; Health Sciences, Immunology; Health Sciences, Pathology

Abstract

A variety of highly sensitive laboratory techniques is now available for the detection and localization of infectious agents in the placenta. This communication discusses, the role of immunohistochemistry, immunofluorescence, in situ nucleic acid hybridization, and polymerase chain reaction for infectious disease diagnosis in formalinfixed placental tissues. These techniques, as well as other sophisticated molecular methods currently in development, will greatly facilitate characterizing the role of infectious agents in a variety of perinatal and pediatric conditions, including birth defects, intrauterine growth retardation, and stillbirth. © 1994, University of Rochester Press. All rights reserved.