Human Suction Blister Fluid Composition Determined Using High-Resolution Metabolomics

• Type of Material: Article
• Authors: Megan M. Niedzwiecki, Emory University; Pradnya Samant, Georgia Institute of Technology; Douglas Walker, Emory University; Tran ViLinh, Emory University; Dean P Jones, Emory University; Mark R. Prausnitz, Emory University; Gary W Miller, Emory University
• Language: English
• Date: 2018-03-20
• Publisher: American Chemical Society
• Publication Version: Final Published Version
• Copyright Statement: © 2018 American Chemical Society. This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
• Final Published Version (URL): http://dx.doi.org/10.1021/acs.analchem.7b04073
• Title of Journal or Parent Work: Analytical Chemistry
• ISSN: 0003-2700
• Volume: 90
• Issue: 6
• Start Page: 3786
• End Page: 3792
• Grant/Funding Information: This work was supported by grants P30 ES019776, U2C ES026560, and T32 ES012870-13 from the National Institutes of Health.
• Keywords: POINT-OF-CARE; UROCANIC ACID; PROTEOMIC CHARACTERIZATION; DERMAL INTERSTITIAL FLUID; DISCOVERY; HUMAN-SKIN; CANCER; Chemistry; Chemistry, Analytical; Physical Sciences; BIOMARKERS; Science & Technology; MASS-SPECTROMETRY; CARDIOVASCULAR-DISEASE
• Research Categories: Engineering, Biomedical; Health Sciences, Public Health

Abstract

Interstitial fluid (ISF) surrounds the cells and tissues of the body. Since ISF has molecular components similar to plasma, as well as compounds produced locally in tissues, it may be a valuable source of biomarkers for diagnostics and monitoring. However, there has not been a comprehensive study to determine the metabolite composition of ISF and to compare it to plasma. In this study, the metabolome of suction blister fluid (SBF), which largely consists of ISF, collected from 10 human volunteers was analyzed using untargeted high-resolution metabolomics (HRM). A wide range of metabolites were detected in SBF, including amino acids, lipids, nucleotides, and compounds of exogenous origin. Various systemic and skin-derived metabolite biomarkers were elevated or found uniquely in SBF, and many other metabolites of clinical and physiological significance were well correlated between SBF and plasma. In sum, using untargeted HRM profiling, this study shows that SBF can be a valuable source of information about metabolites relevant to human health.