Raster-scanning Donut simplifies MINFLUX and provides alternative implement on other scanning-based microscopes

• Type of Material: Article
• Authors: Xinzhu Xu, Peking University; Shu Jia, Emory University; Peng Xi, Peking University
• Language: English
• Date: 2022-10-10
• Publisher: SPRINGERNATURE
• Publication Version: Final Published Version
• Copyright Statement: © The Author(s) 2022
• License: Creative Commons Attribution 4.0 International
• Final Published Version (URL): http://dx.doi.org/10.1038/s41377-022-00983-6
• Title of Journal or Parent Work: LIGHT-SCIENCE & APPLICATIONS
• Volume: 11
• Issue: 1
• Start Page: 293
• End Page: 293
• Keywords: LIMIT; Physical Sciences; Science & Technology; Optics
• Research Categories: Engineering, Biomedical

Abstract

A donut excitation moves around a single molecule with a zigzag configuration lattice by lattice. Such a method implemented in scanning fluorescence microscopy simplifies the conventional MINFLUX process. Consisting of hollow zero-intensity excitation, single-pixel detection, time-correlated single photon counting, and drift stabilization, the system achieves localization precision and resolution very close to conventional MINFLUX theoretically and experimentally. An averaged high-SNR reference, and pixel-registered intensity from a single molecule is essential to reconstruct localization in maximum likelihood estimation. With performance reaching nearly conventional MINFLUX’s, the proposed raster-scanning MINFLUX can inspire researchers expertized in STED or confocal setup to quickly transform to MINFLUX and develop for further exploring on bio-specimens or optical applications.