Publication

Increased Antibody Response to Fucosylated Oligosaccharides and Fucose-Carrying Bacteroides Species in Crohn’s Disease

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  • 05/14/2025
Type of Material
Authors
    Katharina Kappler, Emory UniversityYi Lasanajak, Emory UniversityDavid Smith, Emory UniversityLennart Opitz, University of ZurichThierry Hennet, University of Zurich
Language
  • English
Date
  • 2020-07-14
Publisher
  • Frontiers Media
Publication Version
Copyright Statement
  • © 2020 Kappler, Lasanajak, Smith, Opitz and Hennet.
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Final Published Version (URL)
Title of Journal or Parent Work
Volume
  • 11
Grant/Funding Information
  • This work was supported by the Swiss National Foundation grant 314730_172880 to TH and in part by the Emory Comprehensive Glycomics Core (ECGC). Additional support was provided by the National Center for Advancing Translational Sciences of the National Institutes of Health under Award Number UL1TR000454. The content is solely the responsibility of the authors and does not necessarily reflect the official views of the National Institutes of Health.
  • The serum samples were provided by the Swiss IBD cohort, supported by the Swiss National Foundation grant 33CS30_177523. This study was supported in part by the Emory Comprehensive Glycomics Core (ECGC), which is subsidized by the Emory University School of Medicine and is one of the Emory Integrated Core Facilities. Additional support was provided by the National Center for Advancing Translational Sciences of the National Institutes of Health under Award Number UL1TR000454.
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Abstract
  • Inflammatory bowel disease is associated with intestinal dysbiosis and with elevated antibody production toward microbial epitopes. The underlying processes linking the gut microbiota with inflammation are still unclear. Considering the constant induction of antibodies by gut microbial glycans, the aim of this study was to address whether the repertoire of carbohydrate-specific antibodies is altered in Crohn’s disease or ulcerative colitis. IgG and IgM reactivities to oligosaccharides representative of mucosal glycans were tested in blood serum from 20 healthy control subjects, 17 ulcerative colitis patients, and 23 Crohn’s disease patients using glycan arrays. An increased IgG and IgM reactivity toward fucosylated oligosaccharides was detected in Crohn’s disease but not in ulcerative colitis. To address the antibody reactivity to the gut microbiota, IgG binding to members of a complex intestinal microbiota was measured and observed to be increased in sera of patients with Crohn’s disease. Based on the elevated reactivity to fucosylated oligosaccharides, gut bacteria were tested for recognition by the fucose-binding Aleuria aurantia lectin. Bacteroides stercoris was detected in IgG- and lectin-positive fractions and reactivity of A. aurantia lectin was demonstrated for additional Bacteroides species. IgG reactivity to these Bacteroides species was significantly increased in inflammatory bowel disease patients, indicating that the increased reactivity to fucosylated oligosaccharides detected in Crohn’s disease may be induced by fucose-carrying intestinal bacteria. Enhanced antibody response to fucosylated epitopes may have systemic effects by altering the binding of circulating antibodies to endogenous glycoproteins.
Author Notes
Keywords
Research Categories
  • Health Sciences, Pathology
  • Biology, Microbiology
  • Biology, Cell

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