Mobile Elements and Chromosomal Changes Associated with MLS Resistance Phenotypes of Invasive Pneumococci Recovered in the United States

• Type of Material: Article
• Authors: Paulina A. Hawkins, Emory University; Sopio Chochua, Emory University; Delois Jackson, Centers for Disease Control and Prevention; Bernard Beall, Centers for Disease Control and Prevention; Lesley McGee, Emory University
• Language: English
• Date: 2015-04
• Publisher: Mary Ann Liebert
• Publication Version: Author Accepted Manuscript (After Peer Review)
• Copyright Statement: © 2015, Mary Ann Liebert, Inc.
• Final Published Version (URL): http://dx.doi.org/10.1089/mdr.2014.0086
• Title of Journal or Parent Work: Microbial Drug Resistance
• ISSN: 1076-6294
• Volume: 21
• Issue: 2
• Start Page: 121
• End Page: 129
• Research Categories: Health Sciences, Medicine and Surgery; Health Sciences, Public Health

Abstract

Pneumococcal macrolide resistance is usually expressed as one of two phenotypes: the M phenotype conferred by the mef gene or the MLSB phenotype caused by modification of ribosomal targets, most commonly mediated by an erm methylase. Target-site modification leading to antibiotic resistance can also occur due to sequence mutations within the 23S rRNA or the L4 and L22 riboproteins. We screened 4,535 invasive isolates resistant to erythromycin and 18 invasive isolates nonsusceptible to quinupristin–dalfopristin (Q-D) to deduce the potential mechanisms involved. Of 4,535 erythromycin-resistant isolates, 66.2% were polymerase chain reaction (PCR)-positive for mef alone, 17.8% for ermB alone, and 15.1% for both mef and ermB. Thirty-seven isolates (0.9%) were PCR negative for both determinants. Of these, 3 were positive for ermA (subclass ermTR) and 25 had chromosomal mutations. No chromosomal mutations (in 23S rRNA, rplD, or rplV) nor any of the macrolides/lincosamides/streptogramin (MLS) resistance genes screened for (ermT, ermA, cfr, lsaC, and vgaA) were found in the remaining nine isolates. Of 18 Q-D nonsusceptible isolates, 14 had chromosomal mutations and one carried both mef and ermB; no chromosomal mutations or other resistance genes were found in 3 isolates. Overall, we found 28 mutations, 13 of which have not been previously described in Streptococcus pneumoniae. The role of these mutations remains to be confirmed by transformation assays.