Cryopreservation of Transgenic Huntington’s Disease Rhesus Macaque Sperm: A Case Report

• Type of Material: Article
• Authors: Kittiphong Putkhao, Emory University; Anthony Chan, Emory University; Yuksel Agca, Suranaree University of Technology; Rangsun Parnpai, Emory University
• Language: English
• Date: 2013
• Publisher: OMICS Publishing Group
• Publication Version: Final Published Version
• Copyright Statement: © 2013 Putkhao K, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
• Final Published Version (URL): http://dx.doi.org/10.4172/2168-9849.1000116
• Title of Journal or Parent Work: Cloning and Transgenesis
• ISSN: 2168-9849
• Volume: 02
• Issue: 03
• Grant/Funding Information: The YNPRC is supported by the base grant P51RR165 awarded by the Animal Resources Program of the NIH.; This work was supported by ORIP/NIH (RR018827), and in part by the Royal Golden Jubilee Ph.D program of Thailand Research Fund in cooperation with Suranaree University of Technology. AWSC is supported by the ORIP/NIH (RR018827), NINDS (NS064991) and the American Recovery and Reinvestment Act (ARRA) Fund.
• Keywords: Transgenic monkey model; Sperm cryopreservation; Freezing extender; Huntington's disease
• Research Categories: Health Sciences, General

Abstract

The cryoprotective effects of glycerol in three different semen freezing extenders, Tris-citrate (TRIS), TEST, and Tes-Tris-Egg yolk (TTE), on wild-type (WT) rhesus monkey (Macaca mulatta) sperm cryopreservation have been tested. Sperm motility and viability were examined to evaluate the integrity of frozen-thawed sperm, and the best extender was selected to cryopreserve sperm from transgenic Huntington's disease (HD) rhesus monkey. The results showed no post-thaw motility difference among the freezing extender tested (P>0.05). However, sperm membrane integrity in TEST and TTE were significantly better than in TRIS extender (P<0.05). TEST was chosen for HD rhesus monkey sperm cryopreservation. The results showed that post-thawed HD sperm motility and viability was not different compared with WT control group (P>0.05). The present study demonstrates that TEST and TTE were excellent extenders and suitable for rhesus monkey sperm cryopreservation and no detectible differences of post-thaw sperm motility and viability between HD and WT rhesus monkeys resulted from TEST extender.