Protective Effects of CISD2 and Influence of Curcumin on CISD2 Expression in Aged Animals and Inflammatory Cell Model

• Type of Material: Article
• Authors: Chai-Ching Chiang, National Ilan University; Yu-Yo Sun, Emory University; Chai-Ching Lin, National Ilan University; Muh-Shi Lin, National Ilan University
• Language: English
• Date: 2019-03-25
• Publisher: MDPI
• Publication Version: Final Published Version
• Copyright Statement: © 2019 by the authors. Licensee MDPI, Basel, Switzerland.
• Final Published Version (URL): http://dx.doi.org/10.3390/nu11030700
• Title of Journal or Parent Work: Nutrients
• ISSN: 2072-6643
• Volume: 11
• Issue: 3
• Grant/Funding Information: This work was supported by grants from Kuang Tien General Hospital, Taiwan (Kuang Tien 105-07 and 106-12).; This research received no external funding.
• Keywords: INJURY; IN-VIVO; neurodegeneration; aging; MITOCHONDRIAL DYSFUNCTION; Science & Technology; Nutrition & Dietetics; HOMEOSTASIS; ALZHEIMER; AUTOPHAGY; ASTROCYTES; curcumin; BRAIN; MECHANISMS; CISD2-dependent manner; trauma; Life Sciences & Biomedicine; CISD2
• Research Categories: Biology, Neuroscience; Biology, Cell; Health Sciences, Medicine and Surgery

Abstract

Background: Inflammation and mitochondrial dysfunction have been linked to trauma, neurodegeneration, and aging. Impairment of CISD2 expression may trigger the aforementioned pathological conditions in neural cells. We previously reported that curcumin attenuates the downregulation of CISD2 in animal models of spinal cord injury and lipopolysaccharide (LPS)-treated neuronal cells. In this study, we investigate (1) the role of CISD2 and (2) how curcumin regulates CISD2 in the aging process. Materials and methods: The serial expression of CISD2 and the efficacy of curcumin treatment were evaluated in old (104 weeks) mice and long-term cultures of neural cells (35 days in vitro, DIV). LPS-challenged neural cells (with or without siCISD2 transfection) were used to verify the role of curcumin on CISD2 underlying mitochondrial dysfunction. Results: In the brain and spinal cord of mice aged P2, 8, 25, and 104 weeks, we observed a significant decrease in CISD2 expression with age. Curcumin treatment in vivo and in vitro was shown to upregulate CISD2 expression; attenuate inflammatory response in neural cells. Moreover, curcumin treatment elevated CISD2 expression levels and prevented mitochondrial dysfunction in LPS-challenged neural cells. The beneficial effects of curcumin in either non-stressed or LPS-challenged cells that underwent siCISD2 transfection were significantly lower than in respective groups of cells that underwent scrambled siRNA-transfection. Conclusions: We hypothesize that the protective effects of curcumin treatment in reducing cellular inflammation associated trauma, degenerative, and aging processes can be partially attributed to elevated CISD2 expression. We observed a reduction in the protective effects of curcumin against injury-induced inflammation and mitochondrial dysfunction in cells where CISD2 expression was reduced by siCISD2.