Decrease in Formalin-Inactivated Respiratory Syncytial Virus (FI-RSV) Enhanced Disease with RSV G Glycoprotein Peptide Immunization in BALB/c Mice

• Type of Material: Article
• Authors: Gertrud U. Rey, Centers for Disease Control and Prevention; Congrong Miao, Centers for Disease Control and Prevention; Hayat Caidi, Centers for Disease Control and Prevention; Suvang U. Trivedi, Centers for Disease Control and Prevention; Jennifer L. Harcourt, Centers for Disease Control and Prevention; Ralph A. Tripp, University of Georgia; Larry Anderson, Emory University; Lia M. Haynes, Centers for Disease Control and Prevention
• Language: English
• Date: 2013-12-23
• Publisher: Public Library of Science
• Publication Version: Final Published Version
• Copyright Statement: This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
• License: Creative Commons Creative Commons CC0 1.0 Universal
• Final Published Version (URL): http://dx.doi.org/10.1371/journal.pone.0083075
• Title of Journal or Parent Work: PLoS ONE
• ISSN: 1932-6203
• Volume: 8
• Issue: 12
• Start Page: e83075
• End Page: e83075
• Grant/Funding Information: This research was supported in part by an appointment to the Research Participation Program at the Centers for Disease Control and Prevention (CDC) administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement between the U.S. Department of Energy and CDC.; This research was also supported in part by the National Institutes of Health (5RO1AI06275-03) and through the Georgia Research Alliance to R.T.; G.R. and SUT were ORISE Fellows at the time this study was performed.
• Keywords: MULTIDISCIPLINARY SCIENCES; INFECTION; T-CELLS; EOSINOPHILIA; YOUNG-CHILDREN; MESSENGER-RNA EXPRESSION; IMMUNOGENICITY; Multidisciplinary Sciences; PULMONARY INFLAMMATORY RESPONSE; Science & Technology - Other Topics; ATTACHMENT G PROTEIN; Science & Technology; SUBSTANCE-P; VACCINE
• Research Categories: Biology, Veterinary Science; Health Sciences, Immunology

Abstract

Respiratory syncytial virus (RSV) is a high priority target for vaccine development. One concern in RSV vaccine development is that a non-live virus vaccine would predispose for enhanced disease similar to that seen with the formalin inactivated RSV (FI-RSV) vaccine. Since a mAb specific to RSV G protein can reduce pulmonary inflammation and eosinophilia seen after RSV infection of FI-RSV vaccinated mice, we hypothesized that RSV G peptides that induce antibodies with similar reactivity may limit enhanced disease after subunit or other non-live RSV vaccines. In support of this hypothesis, we show that FI-RSV vaccinated mice administered RSV G peptide vaccines had a significant reduction in enhanced disease after RSV challenge. These data support the importance of RSV G during infection to RSV disease pathogenesis and suggest that use of appropriately designed G peptide vaccines to reduce the risk of enhanced disease with non-live RSV vaccines merits further study.