MHC II on Transfused Murine Blood is Not Required for Alloimmunization Against MHC I

• Type of Material: Article
• Authors: Christopher Raleigh Gilson, Emory University; Chantel M. Cadwell, Emory University; Nicole H. Smith, Emory University; Jeanne Hendrickson, Emory University; James C Zimring, Emory University
• Language: English
• Date: 2010-11
• Publisher: Wiley: 12 months
• Publication Version: Author Accepted Manuscript (After Peer Review)
• Copyright Statement: © 2010 The Author(s). Vox Sanguinis © 2010 International Society of Blood Transfusion
• Final Published Version (URL): http://onlinelibrary.wiley.com/doi/10.1111/j.1423-0410.2010.01351.x/abstract
• Title of Journal or Parent Work: Vox Sanguinis
• ISSN: 0042-9007
• Volume: 99
• Issue: 4
• Start Page: 369
• End Page: 374
• Grant/Funding Information: National Heart, Lung, and Blood Institute : NHLBI; These studies were supported in part by a grant from the N.I.H. to J.C.Z. (P01 HL086773-project 4)
• Keywords: alloimmunization; MHC class II; Transfusion
• Research Categories: Health Sciences, Pathology

Abstract

Background and Objectives Transfusion of allogeneic platelet products can result in antibodies against donor MHC I antigens, leading to a refractory state to subsequent platelet transfusions. However, there is disagreement in the field regarding the molecular mechanisms of humoral alloimmunization. One hypothesis states that donor MHC II is a requirement for alloimmunization. However, other studies have suggested that donor MHC I is alone sufficient and MHC II is not required. Materials and Methods We utilized a mouse model of anti-MHC I alloimmunization to transfused blood, which employed donors with a complete deletion of all MHC II genes. BALB/c (H-2d) recipients were transfused with blood from either C57BL/6 (H-2b) or MHC II null donors on a C57BL/6 background. Anti-MHC I alloimmunization was monitored by indirect immunofluorescence. Results Recipients of either wild type or MHC II null blood produced equivalent humoral responses against donor MHC I antigens. However, there was variation in the relative amounts of IgG subclasses. Conclusion These data reject the hypothesis that donor MHC II expression is required for alloimmunization to MHC I antigens.