Comparison of Heat Inactivation and Cell Disruption Protocols for Identification of Mycobacteria from Solid Culture Media by Use of Vitek Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

• Type of Material: Article
• Authors: Alexandra Machen, Emory University; Miwako Kobayashi, Emory University; Mary Robin Connelly, Georgia Public Health Laboratory; Yun F (Wayne) Wang, Emory University
• Language: English
• Date: 2013-12
• Publisher: American Society for Microbiology
• Publication Version: Final Published Version
• Copyright Statement: © 2013, American Society for Microbiology. All Rights Reserved.
• Final Published Version (URL): http://jcm.asm.org/content/51/12/4226
• Title of Journal or Parent Work: Journal of Clinical Microbiology
• ISSN: 0095-1137
• Volume: 51
• Issue: 12
• Start Page: 4226
• End Page: 4229
• Grant/Funding Information: No financial support was provided for performance of the study.
• Research Categories: Biology, Microbiology; Health Sciences, General

Abstract

Two novel protocols for inactivation and extraction were developed and used to identify 107 Mycobacterium clinical isolates, including Mycobacterium tuberculosis complex, from solid cultures using Vitek matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectrometry. The protocol using heat inactivation with sonication and cell disruption with glass beads resulted in 82.2% and 88.8% species and genus level identifications, respectively.