Diapause is associated with a change in the polarity of secretion of insulin-like peptides

• Type of Material: Article
• Authors: Yohei Matsunaga, Tottori University; Yoko Honda, Tokyo Metropolitan Institute of Gerontology; Shuji Honda, Tokyo Metropolitan Institute of Gerontology; Takashi Iwasaki, Tottori University; Hiroshi Qadota, Emory University; Guy Benian, Emory University; Tsuyoshi Kawano, Tottori University
• Language: English
• Date: 2016-02-01
• Publisher: Nature Publishing Group
• Publication Version: Final Published Version
• Copyright Statement: © 2016, Nature Publishing Group, a division of Macmillan Publishers Limited.
• License: Creative Commons Attribution 4.0 International
• Final Published Version (URL): http://dx.doi.org/10.1038/ncomms10573
• Title of Journal or Parent Work: Nature Communications
• ISSN: 2041-1723
• Volume: 7
• Start Page: 10573
• End Page: 10573
• Grant/Funding Information: This work was supported by JSPS KAKENHI Grant Numbers 23580150 (to T.K.) and 26292061 (to T.K.).
• Keywords: Multidisciplinary Sciences; CAENORHABDITIS-ELEGANS; DAF-2; C-ELEGANS; EXPRESSION PATTERN; Science & Technology; LIFE-SPAN; PHYSIOLOGICAL-FUNCTION; Science & Technology - Other Topics; GENE; LONGEVITY; EPITHELIAL-CELLS; SENSORY INFORMATION
• Research Categories: Biology, Physiology; Biology, Genetics

Abstract

The insulin/IGF-1 signalling (IIS) pathway plays an important role in the regulation of larval diapause, the long-lived growth arrest state called dauer arrest, in Caenorhabditis elegans. In this nematode, 40 insulin-like peptides (ILPs) have been identified as putative ligands of the IIS pathway; however, it remains unknown how ILPs modulate larval diapause. Here we show that the secretory polarity of INS-35 and INS-7, which suppress larval diapause, is changed in the intestinal epithelial cells at larval diapause. These ILPs are secreted from the intestine into the body cavity during larval stages. In contrast, they are secreted into the intestinal lumen and degraded during dauer arrest, only to be secreted into the body cavity again when the worms return to developmental growth. The process that determines the secretory polarity of INS-35 and INS-7, thus, has an important role in the modulation of larval diapause.