Publication

Proteomic identification of novel proteins associated with Lewy bodies

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Last modified
  • 02/20/2025
Type of Material
Authors
    Qiangwei Xia, Emory UniversityLujian Liao, Emory UniversityDongmei Cheng, Emory UniversityDuc Duong, Emory UniversityMarla Gearing, Emory UniversityJames J Lah, Emory UniversityAllan I Levey, Emory UniversityJunmin Peng, Emory University
Language
  • English
Date
  • 2008-05-01
Publisher
  • Frontiers in Bioscience
Publication Version
Copyright Statement
  • © Frontiers in Bioscience, 1995
Final Published Version (URL)
Title of Journal or Parent Work
ISSN
  • 1093-9946
Volume
  • 13
Start Page
  • 3850
End Page
  • 3856
Grant/Funding Information
  • This work was partially supported by NIH grants ES012068, and the Emory Alzheimer’s Disease Center AG025688.
Abstract
  • The manifestation of Lewy bodies (LB) in the brain is a hallmark of Parkinson’s disease. Here, we present a comprehensive analysis of protein elements in Lewy bodies by comparative mass spectrometry. Cortical LB inclusions were enriched by sucrose gradient centrifugation from postmortem brains, and a negative control sample was prepared from specimen without LB pathology. Whereas ~550 proteins were identified in the LB-enriched sample by mass spectrometry, quantitative comparison with the control sample revealed that ~40 proteins were co-enriched with α-synuclein, the major component in Lewy bodies. As expected, the list of proteins included previously reported constituents, such as those involved in protein folding, membrane trafficking and oxidative stress. More interestingly, we discovered in the LB-enriched sample several kinases (MAPKK1/MEK1, protein kinase C, and doublecortin-like kinase), a novel deubiquitinating enzyme (otubain 1), and numerous ubiquitin ligases (KPC and SCF). The proteomic studies provide enzyme candidates to investigate the regulation of α-synuclein and/or other LB proteins, which may contribute to the formation of Lewy bodies and the toxicity of α-synuclein in the related neurodegenerative disorders.
Author Notes
  • Send correspondence to: Dr. Junmin Peng, Department of Human Genetics, Emory University School of Medicine, Atlanta, Georgia 30322 Tel: 404-712-8510, Fax: 404-727-3728, E-mail: jpeng@genetics.emory.edu
Keywords
Research Categories
  • Biology, Neuroscience
  • Health Sciences, Pathology

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