A Role for Cargo in Arf-dependent Adaptor Recruitment

• Type of Material: Article
• Authors: Amanda H. Caster, Emory University; Elizabeth Sztul, University of Alabama at Birmingham; Richard A Kahn, Emory University
• Language: English
• Date: 2013-05-24
• Publisher: American Society for Biochemistry and Molecular Biology
• Publication Version: Final Published Version
• Copyright Statement: © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.
• Final Published Version (URL): http://www.jbc.org/content/288/21/14788
• Title of Journal or Parent Work: Journal of Biological Chemistry
• ISSN: 0021-9258
• Volume: 288
• Issue: 21
• Start Page: 14788
• End Page: 14804
• Grant/Funding Information: This work was supported, in whole or in part, by National Institutes of Health Grant GM067226 (NIGMS) and by Imaging Core of the Emory Neuroscience NINDS Core Facilities Grant P30NS055077.
• Keywords: Mannose 6-Phosphate Receptor; Arf; AP-1; GGA; Endosomes; Golgi; Furin; Adaptor Proteins; Membrane Trafficking
• Research Categories: Health Sciences, General; Chemistry, Biochemistry; Biology, Molecular

Abstract

Background: Cargo proteins recruit Arf-dependent adaptors for packaging. Results: Cargo presence at the Golgi/endosomes leads to specific adaptor recruitment. Conclusion: Arf activation is more closely coupled to cargo than previously appreciated, and we interpret the specificity in adaptor recruitment as evidence of the lack of freely diffusible activated Arfs. Significance: Cargos play a role in the activation of Arfs and recruitment of specific adaptors.