Metallomics in pulmonary arterial hypertension patients

• Type of Material: Article
• Authors: Karim El-Kersh, University of Nebraska Medical Center; Danielle C Hopkins, University of Louisville; Xiaoyong Wu, University of Cincinnati; Shesh N Rai, University of Cincinnati; Matthew C Cave, University of Louisville; Matthew Smith, Emory University; Young-Mi Go, Emory University; Dean Jones, Emory University; Lu Cai, University of Louisville; Jiapeng Huang, University of Louisville
• Language: English
• Date: 2023-01-01
• Publisher: WILEY
• Publication Version: Final Published Version
• Copyright Statement: © 2023 The Authors. Pulmonary Circulation published by John Wiley & Sons Ltd on behalf of Pulmonary Vascular Research Institute.
• License: Creative Commons Attribution-NonCommercial 4.0 International
• Final Published Version (URL): http://dx.doi.org/10.1002/pul2.12202
• Title of Journal or Parent Work: PULMONARY CIRCULATION
• Volume: 13
• Issue: 1
• Start Page: e12202
• End Page: e12202
• Keywords: silver; RATS; pulmonary hypertension; Cardiac & Cardiovascular Systems; Life Sciences & Biomedicine; Science & Technology; INJURY; metal; SERUM COPPER CONCENTRATION; Cardiovascular System & Cardiology; SILVER NANOPARTICLES; chromium; copper; Respiratory System

Abstract

Pulmonary arterial hypertension (PAH) prevalence is increasing worldwide, and the prognosis is poor with 5-year survival < 50% in high risk patients. The relationship between metal exposure/essential metal dyshomeostasis and PAH/right ventricular dysfunction is less investigated. The aim of this study is to investigate vegetable consumptions and metal levels between PAH patients and controls. This was a prospective, single center pilot study. Questionnaires were completed by all study subjects (20 PAH patients and 10 healthy controls) on smoking, metal exposure risks, metal supplements, and vegetable consumptions. Blood and urine samples were collected to measure 25 metal levels in blood, plasma, and urine using an X Series II quadrupole inductively coupled plasma mass spectrometry. Statistical analysis was conducted using SAS 9.5 and results with p value < 0.05 were considered significant. Vegetables consumptions (broccoli risk ratio [RR] = 0.4, CI = (0.2, 0.9)], cabbage [RR = 0.2, CI = (0.1, 0.8)], and brussel sprouts [RR = 0.2, CI = (0.1, 0.5)]) are associated with less risks of PAH. In the plasma samples, silver (p < 0.001), and copper (p = 0.002) levels were significantly higher in PAH patients. There was significant positive correlation between cardiac output and cardiac index with plasma levels of silver (r = 0.665, p = 0.001 and r = 0.678 p = 0.001), respectively. There was significant correlation between mixed venous saturation, 6-min walk distance, and last BNP with plasma levels of chromium (r = −0.520, p = 0.022; r = −0.55, p = 0.014; r = 0.463, p = 0.039), respectively. In conclusion, there are significant differences between PAH and control groups in terms of vegetable consumptions and metal concentrations. Silver and chromium levels are correlated with clinical indicators of PAH severities.