Expression and function of NIK- and IKK2-binding protein (NIBP) in mouse enteric nervous system

• Type of Material: Article
• Authors: Yonggang Zhang, Temple University; Daniel Bitner, Temple University; Adalto Alfredo Pontes Filho, Temple University; Fang Li, Temple University; Shu Liu, Temple University; Hong Wang, Temple University; Fan Yang, Temple University; Sam Adhikari, Temple University; Jennifer Gordon, Temple University; Shanthi Srinivasan, Emory University; Wenhui Hu, Temple University
• Language: English
• Date: 2014-01-01
• Publisher: Wiley: 12 months
• Publication Version: Author Accepted Manuscript (After Peer Review)
• Copyright Statement: © 2013 John Wiley & Sons Ltd.
• Final Published Version (URL): http://dx.doi.org/10.1111/nmo.12234
• Title of Journal or Parent Work: Neurogastroenterology and Motility
• ISSN: 1350-1925
• Volume: 26
• Issue: 1
• Start Page: 77
• End Page: 97
• Grant/Funding Information: This work was supported by Grant DK075964 (WH) and Grant DK080684 (SS) from the National Institutes of Diabetes, Digestive, and Kidney Diseases.
• Keywords: Gastroenterology & Hepatology; PLEXUS; Neurosciences & Neurology; Neurosciences; GUT MOTILITY; Clinical Neurology; cytokines; CHOLINERGIC NEURONS; Science & Technology; enteric neuronal cell line; NFB; LARGE-INTESTINE; RECESSIVE INTELLECTUAL DISABILITY; enteric nervous system; NIBP; BETA-BINDING PROTEIN; NITRIC-OXIDE SYNTHASE; immunohistochemistry; Life Sciences & Biomedicine; confocal image; STEM-CELLS; MYENTERIC NEURONS; TRAPPC9; NF-KAPPA-B
• Research Categories: Chemistry, Biochemistry; Biology, Neuroscience

Abstract

Background: NIK- and IKK2-binding protein (NIBP)/TRAPPC9 is expressed in brain neurons, and human NIBP mutations are associated with neurodevelopmental disorders. The cellular distribution and function of NIBP in the enteric nervous system (ENS) remain unknown. Methods: Western blot and reverse transcription-polymerase chain reaction analysis were used respectively to identify the protein and mRNA expression of NIBP and other neuronal markers. Multi-labeled immunofluorescent microscopy and confocal image analysis were used to examine the cellular distribution of NIBP-like immunoreactivity (IR) in whole mount intestine. Enteric neuronal cell line (ENC) was infected with lentivirus carrying NIBP or its shRNA expression vectors and treated with vehicle or tumor necrosis factor (TNF)α. Key Results: NIBP is expressed at both mRNA and protein levels in different regions and layers of the mouse intestine. NIBP-like-IR was co-localized with various neuronal markers, but not with glial, smooth muscular, or interstitial cells of Cajal markers. A small population of NIBP-expressing cells and fibers in extra-ganglionic and intra-ganglionic area were negative for pan-neuronal markers HuD or Peripherin. Relatively high NIBP-like-IR was found in 35-44% of myenteric neurons and 9-10% of submucosal neurons. Approximately 98%, 87%, and 43% of these relatively high NIBP-expressing neurons were positive for choline acetyltransferase, neuronal nitric oxide synthase and Calretinin, respectively. NIBP shRNA knockdown in ENC inhibited TNFα-induced NFκB activation and neuronal differentiation, whereas NIBP overexpression promoted it. Conclusions & Inferences: NIBP is extensively expressed in the ENS with relatively high level in a subpopulation of enteric neurons. Various NIBP expression levels in different neurons may represent dynamic trafficking or posttranslational modification of NIBP in some functionally active neurons and ultimately regulate ENS plasticity.