Publication

Regulatory effects of internn ttent noxious St nnulation on spinal cord injury-sensitive microRNAs and their presumptive targets following spinal cord contusion

Downloadable Content

Persistent URL
Last modified
  • 05/15/2025
Type of Material
Authors
    Eric R. Strickland, Texas A&M Health Science CenterSarah A. Woller, Texas A&M Health Science CenterSandra M. Garraway, Emory UniversityMichelle A. Hook, Texas A&M Health Science CenterJames W. Grau, Texas A&M Health Science CenterRajesh C. Miranda, Texas A&M Health Science Center
Language
  • English
Date
  • 2014-09-18
Publisher
  • Frontiers Media
Publication Version
Copyright Statement
  • © 2014 Strickland, Woller, Garraway, Hook, Grau and Miranda.
License
Final Published Version (URL)
Title of Journal or Parent Work
ISSN
  • 1662-5110
Volume
  • 8
Start Page
  • 117
End Page
  • 117
Grant/Funding Information
  • This work was supported by the National Institute of Health, Grant Number HD058412, and by funds from Texas A&M Health Science Center.
Abstract
  • Uncontrollable nociceptive stimulation adversely affects recovery in spinally contused rats. Spinal cord injury (SCI) results in altered microRNA (miRNA) expression both at, and distal to the lesion site. We hypothesized that uncontrollable nociception further influences SCIsensitive miRNAs and associated gene targets, potentially explaining the progression of maladaptive plasticity. Our data validated previously described sensitivity of miRNAs to SCI alone. Moreover, following SCI, intermittent noxious stimulation decreased expression of miR124 in dorsal spinal cord 24 h after stimulation and increased expression of miR1292 in dorsal, and miR1 in ventral spinal cord at 7 days. We also found that brain-derived neurotrophic factor (BDNF) mRNA expression was significantly down-regulated 1 day after SCI alone, and significantly more so, after SCI followed by tailshock. Insulin-like growth factor-1 (IGF-1) mRNA expression was significantly increased at both 1 and 7 days postSCI, and significantly more so, 7 days post-SCI with shock. MiR1 expression was positively and significantly correlated with IGF-1, but not BDNF mRNA expression. Further, stepwise linear regression analysis indicated that a significant proportion of the changes in BDNF and IGF-1 mRNA expression were explained by variance in two groups of miRNAs, implying co-regulation. Collectively, these data show that uncontrollable nociception which activates sensorimotor circuits distal to the injury site, influences SCI-miRNAs and target mRNAs within the lesion site. SCI-sensitive miRNAs may well mediate adverse consequences of uncontrolled sensorimotor activation on functional recovery. However, their sensitivity to distal sensory input also implicates these miRNAs as candidate targets for the management of SCI and neuropathic pain.
Author Notes
  • Rajesh C. Miranda, Department of Neuroscience and Experimental Therapeutics, College of Medicine, Texas A&M Health Science Center, Medical Research and Education Building, 8447 State Highway 47, Bryan, TX 77807-3260, USA e-mail: miranda@medicine.tamhsc.edu
Keywords
Research Categories
  • Biology, Neuroscience
  • Psychology, General

Tools

Relations

In Collection:

Items

Thumbnail Title File Description Date Uploaded Visibility Actions
file details Publication File - sdnjs.pdf Primary Content 2025-03-15 Public Download