Cell type-specific biotin labeling in vivo resolves regional neuronal and astrocyte proteomic differences in mouse brain

• Type of Material: Article
• Authors: Sruti Rayaprolu, Emory University; Sara Bitarafan, Georgia Institute of Technology; Juliet Santiago, Emory University; Ranjita Betarbet, Emory University; Sydney Sunna, Emory University; Lihong Cheng, Emory University; Hailian Xiao, Emory University; Ruth S Nelson, Emory University; Prateek Kumar, Emory University; Pritha Bagchi, Emory University; Duc M Duong, Emory University; Annie M Goettemoeller, Emory University; Viktor Olah, Emory University; Matt Rowan, Emory University; Allan Levey, Emory University; Levi B Wood, Georgia Institute of Technology; Nicholas Seyfried, Emory University; Srikant Rangaraju, Emory University
• Language: English
• Date: 2022-05-25
• Publisher: NATURE PORTFOLIO
• Publication Version: Final Published Version
• Copyright Statement: © The Author(s) 2022
• License: Creative Commons Attribution 4.0 International
• Final Published Version (URL): http://dx.doi.org/10.1038/s41467-022-30623-x
• Title of Journal or Parent Work: NATURE COMMUNICATIONS
• Volume: 13
• Issue: 1
• Start Page: 2927
• End Page: 2927
• Keywords: MESSENGER-RNA; Multidisciplinary Sciences; ASSOCIATION; Science & Technology - Other Topics; POLYMORPHISM; Science & Technology; PROMOTER; METABOLISM; IDENTIFICATION; GRIN2A; EXPRESSION; PROTEIN; GENE
• Research Categories: Biology, Cell; Chemistry, Biochemistry

Abstract

Proteomic profiling of brain cell types using isolation-based strategies pose limitations in resolving cellular phenotypes representative of their native state. We describe a mouse line for cell type-specific expression of biotin ligase TurboID, for in vivo biotinylation of proteins. Using adenoviral and transgenic approaches to label neurons, we show robust protein biotinylation in neuronal soma and axons throughout the brain, allowing quantitation of over 2000 neuron-derived proteins spanning synaptic proteins, transporters, ion channels and disease-relevant druggable targets. Next, we contrast Camk2a-neuron and Aldh1l1-astrocyte proteomes and identify brain region-specific proteomic differences within both cell types, some of which might potentially underlie the selective vulnerability to neurological diseases. Leveraging the cellular specificity of proteomic labeling, we apply an antibody-based approach to uncover differences in neuron and astrocyte-derived signaling phospho-proteins and cytokines. This approach will facilitate the characterization of cell-type specific proteomes in a diverse number of tissues under both physiological and pathological states.