Integration of machine learning and genome-scale metabolic modeling identifies multi-omics biomarkers for radiation resistance

• Type of Material: Article
• Authors: Joshua E Lewis, Georgia Institute of Technology; Melissa Kemp, Emory University
• Language: English
• Date: 2021-05-11
• Publisher: NATURE PORTFOLIO
• Publication Version: Final Published Version
• Copyright Statement: © The Author(s) 2021
• Final Published Version (URL): http://dx.doi.org/10.1038/s41467-021-22989-1
• Title of Journal or Parent Work: NATURE COMMUNICATIONS
• Volume: 12
• Issue: 1
• Start Page: 2700
• End Page: 2700
• Grant/Funding Information: The authors gratefully acknowledge support for this work from an NIH/NCI F30 CA224968 fellowship (PI: J.E.L.; Sponsor: M.L.K.) and an NIH/NCI U01 CA215848 grant (PI: M.L.K.).
• Keywords: METHYLATION; APOPTOSIS; THERAPY; DNA-DAMAGE; EXPRESSION; Multidisciplinary Sciences; RADIOTHERAPY; HALLMARKS; ASPIRIN; SURVIVAL; Science & Technology - Other Topics; CANCER-CELLS; Science & Technology
• Research Categories: Engineering, Biomedical

Abstract

Resistance to ionizing radiation, a first-line therapy for many cancers, is a major clinical challenge. Personalized prediction of tumor radiosensitivity is not currently implemented clinically due to insufficient accuracy of existing machine learning classifiers. Despite the acknowledged role of tumor metabolism in radiation response, metabolomics data is rarely collected in large multi-omics initiatives such as The Cancer Genome Atlas (TCGA) and consequently omitted from algorithm development. In this study, we circumvent the paucity of personalized metabolomics information by characterizing 915 TCGA patient tumors with genome-scale metabolic Flux Balance Analysis models generated from transcriptomic and genomic datasets. Metabolic biomarkers differentiating radiation-sensitive and -resistant tumors are predicted and experimentally validated, enabling integration of metabolic features with other multi-omics datasets into ensemble-based machine learning classifiers for radiation response. These multi-omics classifiers show improved classification accuracy, identify clinical patient subgroups, and demonstrate the utility of personalized blood-based metabolic biomarkers for radiation sensitivity. The integration of machine learning with genome-scale metabolic modeling represents a significant methodological advancement for identifying prognostic metabolite biomarkers and predicting radiosensitivity for individual patients.