Evaluation of an Immunochromatographic Assay for Rapid Detection of Penicillin-Binding Protein 2a in Human and Animal Staphylococcus intermedius Group, Staphylococcus lugdunensis, and Staphylococcus schleiferi Clinical Isolates

• Type of Material: Article
• Authors: A. R. Arnold, Emory University; C.-A. D. Burnham, Washington University; B. A. Ford, Univ Iowa Hospital and Clinics; S. D. Lawhon, Texas A&M University; S. K. McAllister, Centers for Disease Control and Prevention; D. Lonsway, Centers for Disease Control and Prevention; V. Albrecht, Centers for Disease Control and Prevention; Robert Jerris, Emory University; J. K. Rasheed, Centers for Disease Control and Prevention; B. Limbago, Centers for Disease Control and Prevention; Eileen Burd, Emory University; Lars Westblade, Emory University
• Language: English
• Date: 2016-12-16
• Publisher: American Society for Microbiology
• Publication Version: Final Published Version
• Copyright Statement: © 2016, American Society for Microbiology.
• Final Published Version (URL): http://dx.doi.org/10.1128/JCM.02869-15
• Title of Journal or Parent Work: Journal of Clinical Microbiology
• ISSN: 0095-1137
• Volume: 54
• Issue: 3
• Start Page: 745
• End Page: 748
• Keywords: COAGULASE-NEGATIVE STAPHYLOCOCCI; AUREUS; PATHOGENESIS; PSEUDINTERMEDIUS; DIAGNOSTICS; Science & Technology; Life Sciences & Biomedicine; INFECTION; METHICILLIN RESISTANCE; Microbiology; EMERGENCE; IDENTIFICATION; EPIDEMIOLOGY
• Research Categories: Engineering, Biomedical; Biology, Virology; Health Sciences, General

Abstract

The performance of a rapid penicillin-binding protein 2a (PBP2a) detection assay, the Alere PBP2a culture colony test, was evaluated for identification of PBP2a-mediated beta-lactam resistance in human and animal clinical isolates of Staphylococcus intermedius group, Staphylococcus lugdunensis, and Staphylococcus schleiferi. The assay was sensitive and specific, with all PBP2anegative and PBP2a-positive strains testing negative and positive, respectively.