Arl13b regulates ciliogenesis and the dynamic localization of Shh signaling proteins

• Type of Material: Article
• Authors: Christine E. Larkins, Emory University; Gladys D. Gonzalez Aviles, Emory University; Michael P. East, Emory University; Richard A Kahn, Emory University; Tamara Caspary, Emory University
• Language: English
• Date: 2011-12-01
• Publisher: American Society for Cell Biology
• Publication Version: Final Published Version
• Copyright Statement: © 2011 Larkins et al.
• License: Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported
• Final Published Version (URL): http://www.molbiolcell.org/content/22/23/4694
• Title of Journal or Parent Work: Molecular Biology of the Cell
• ISSN: 1059-1524
• Volume: 22
• Issue: 23
• Start Page: 4694
• End Page: 4703
• Grant/Funding Information: The Viral Vector Core, which generated the Arl13b-GFP lentivirus, and the Microscopy Core of the Emory Neuroscience NINDS Core Facilities were supported by National Institutes of Health (NIH) grant P30-NS055077.; This work was funded by a Predoctoral Fellowship from the Greater Southeast Region American Heart Association (C.E.L.), a Basil O'Conner Starter Scholar Award from the March of Dimes (T.C.), and a research grant from the NIH (R01-NS056380).
• Research Categories: Biology, Genetics

Abstract

We show Arl13b is localized to the ciliary membrane and regulates tubulin modifications and ciliary length in vitro. Significantly, we found that Smoothened is enriched in Arl13b null fibroblasts, even without Sonic hedgehog stimulation, but that Glis are not similarly enriched.