Increased glucose transporter-1 expression on intermediate monocytes from HIV-infected women with subclinical cardiovascular disease

• Type of Material: Article
• Authors: Tiffany R Butterfield, University of the West Indies; David B Hanna, Albert Einstein College of Medicine; Robert C Kaplan, Albert Einstein College of Medicine; Jorge R Kizer, Albert Einstein College of Medicine; Helen G Durkin, Suny Downstate Medical Center; Mary A Young, Georgetown University; Marek J Nowicki, University of Southern California; Phyllis C Tien, University of California San Francisco; Elizabeth T Golub, Johns Hopkins University; Michelle A Floris-Moore, University of North Carolina; Kehmia Titanji, Emory University; Margaret A Fischl, University of Miami; Sonya L Heath, University of Alabama Birmingham; Jefferey Martinson, Rush University; Suzanne M Crowe, Burnet Institute; Clovis S Palmer, Burnet Institute; Alan L Landay, Rush University; Joshua J Anzinger, University of the West Indies
• Language: English
• Date: 2017-01-14
• Publisher: Lippincott, Williams & Wilkins
• Publication Version: Author Accepted Manuscript (After Peer Review)
• Copyright Statement: © 2017 Wolters Kluwer Health, Inc. All rights reserved.
• Final Published Version (URL): http://dx.doi.org/10.1097/QAD.0000000000001320
• Title of Journal or Parent Work: AIDS
• ISSN: 0269-9370
• Volume: 31
• Issue: 2
• Start Page: 199
• End Page: 205
• Grant/Funding Information: Targeted supplemental funding for specific projects is also provided by the National Institute of Dental and Craniofacial Research (NIDCR), the National Institute on Alcohol Abuse and Alcoholism (NIAAA), the National Institute on Deafness and other Communication Disorders (NIDCD), and the NIH Office of Research on Women's Health.; WIHS data collection is also supported by UL1-TR000004 (UCSF CTSA) and UL1-TR000454 (Atlanta CTSA).; The WIHS is funded primarily by the National Institute of Allergy and Infectious Diseases (NIAID), with additional co-funding from the Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD), the National Cancer Institute (NCI), the National Institute on Drug Abuse (NIDA), and the National Institute on Mental Health (NIMH).; The study was supported by the Office of the Principal of the University of the West Indies, Mona and NIH grants U01 AI68636 to the AIDS Clinical Trial Group (A.L.L.) and 1R01HL126543, 1R01HL095140, R21-HL-120394 (R.C.K.).
• Keywords: T-CELL-ACTIVATION; EVENTS; COAGULATION; ATHEROSCLEROSIS; Infectious Diseases; CAROTID-ARTERY-DISEASE; IMMUNE-RESPONSE; cardiovascular disease; T cell; GLUCOSE-METABOLISM; Virology; MARKERS; GLUT1; HIV; Science & Technology; monocyte; INFLAMMATION; INDIVIDUALS; Immunology; Life Sciences & Biomedicine
• Research Categories: Health Sciences, Public Health; Health Sciences, Epidemiology

Abstract

Objective: People living with HIV (PLWH) have chronic immune activation and increased cardiovascular disease (CVD) risk. Activation of monocytes and T lymphocytes causes upregulation of glucose transporter-1 (GLUT1) for efficient function. PLWH have an increased percentage of GLUT1-expressing monocytes and T lymphocytes, but it is unclear if these cells are associated with CVD. We evaluated the expression of GLUT1 and CD38 on monocyte and T lymphocyte populations from HIVinfected women with subclinical CVD. Methods: Participants with more than 75th percentile (n-15) and less than 25th percentile (n=15) age-Adjusted intima-media thickness (IMT) at the right common carotid artery and bifurcation were identified from the Women's Interagency HIV Study. Groups were matched by age, race/ethnicity, smoking status, and CD4 cell count. All women were receiving suppressive antiretroviral therapy except for one high and one low IMT participant. Monocyte and T lymphocyte populations were evaluated for GLUT1 and CD38 expression using flow cytometry. Results: Intermediate monocytes from high IMT women had significantly increased expression of GLUT1 (310 MFI vs. 210 MFI, P=0.024) (66.4% vs. 48.5%, P=0.031) and CD38 (339MFI vs. 211MFI, P=0.002) (10.5%vs. 3.8%, P=0.0002) compared withwomen with lowIMT. High and lowIMT participants showed no differences inGLUT1 or CD38 expression on classical monocytes, nonclassical monocytes, CD4 and CD8 T lymphocytes. Conclusion: GLUT1-expressing intermediate monocytes are elevated in HIV-infected women with subclinical CVD. These cells may contribute to development of CVD in PLWH and could be a novel target to limit inflammation.