A Dynamic Database of Microarray-Characterized Cell Lines with Various Cytogenetic and Genomic Backgrounds

• Type of Material: Article
• Authors: Zhenya Tang, Coriell Institute for Medical Research; Dorit S. Berlin, Coriell Institute for Medical Research; Lorraine Toji, Coriell Institute for Medical Research; Gokce A. Toruner, New Jersey Medical School; Christine Beiswanger, Coriell Institute for Medical Research; Shashikant Kulkarni, Washington University St. Louis; Christa Martin, Emory University; Beverly S. Emanuel, University of Pennsylvania; Michael Christman, Coriell Institute for Medical Research; Norman P. Gerry, Coriell Institute for Medical Research
• Language: English
• Date: 2013-07-01
• Publisher: Genetics Society of America
• Publication Version: Final Published Version
• Copyright Statement: © 2013 Tang et al.
• License: Creative Commons Attribution 3.0 Unported
• Final Published Version (URL): http://dx.doi.org/10.1534/g3.113.006577
• Title of Journal or Parent Work: G3 : Genes, Genomes, Genetics
• Volume: 3
• Issue: 7
• Start Page: 1143
• End Page: 1149
• Grant/Funding Information: The NIGMS Repository, sponsored by the National Institute of General Medical Sciences, is supported by contract no. HHS-N-263-2009-00026C.
• Keywords: karyotype; FISH; chromosomal microarray analysis; ISCN 2013
• Research Categories: Biology, Genetics; Health Sciences, Pathology

Abstract

The Human Genetic Cell Repository sponsored by the National Institute of General Medical Sciences (NIGMS) contains more than 11,000 cell lines and DNA samples collected from numerous individuals. All of these cell lines and DNA samples are categorized into several collections representing a variety of disease states, chromosomal abnormalities, heritable diseases, distinct human populations, and apparently healthy individuals. Many of these cell lines have previously been studied with detailed conventional cytogenetic analyses, including G-banded karyotyping and fluorescence in situ hybridization. This work was conducted by investigators at submitting institutions and scientists at Coriell Institute for Medical Research, where the NIGMS Repository is hosted. Recently, approximately 900 cell lines, mostly chosen from the Chromosomal Aberrations and Heritable Diseases collections, have been further characterized in detail at the Coriell Institute using the Affymetrix Genome-Wide Human SNP Array 6.0 to detect copy number variations and copy number neutral loss of heterozygosity. A database containing detailed cytogenetic and genomic information for these cell lines has been constructed and is freely available through several sources, such as the NIGMS Repository website and the University of California at Santa Cruz Genome Browser. As additional cell lines are analyzed and subsequently added into it, the database will be maintained dynamically.