Live-Cell Imaging of Early Steps of Single HIV-1 Infection

• Type of Material: Article
• Authors: Ashwanth Francis, Emory University; Gregory Melikian, Emory University
• Language: English
• Date: 2018-05-01
• Publisher: MDPI
• Publication Version: Author Accepted Manuscript (After Peer Review)
• Copyright Statement: © 2018 by the authors. Licensee MDPI, Basel, Switzerland.
• License: Creative Commons Attribution 4.0 International
• Final Published Version (URL): http://dx.doi.org/10.3390/v10050275
• Title of Journal or Parent Work: Viruses
• ISSN: 1999-4915
• Volume: 10
• Issue: 5
• Grant/Funding Information: This work was supported by the NIH R01 GM054787 and AI129862 grants to G.B.M.
• Keywords: viral fusion; Single HIV imaging; uncoating; REAL-TIME; Virology; nuclear import; T-CELLS; infection; VISUALIZATION; FUSION; VIROLOGICAL SYNAPSES; VIRAL PARTICLES; CYCLOPHILIN-A; NUCLEAR IMPORT; maturation; IMMUNODEFICIENCY-VIRUS TYPE-1; GAG; Science & Technology; Life Sciences & Biomedicine
• Research Categories: Health Sciences, Medicine and Surgery; Biology, Virology

Abstract

Live-cell imaging of single HIV-1 entry offers a unique opportunity to delineate the spatio-temporal regulation of infection. Novel virus labeling and imaging approaches enable the visualization of key steps of HIV-1 entry leading to nuclear import, integration into the host genome, and viral protein expression. Here, we discuss single virus imaging strategies, focusing on live-cell imaging of single virus fusion and productive uncoating that culminates in HIV-1 infection.