Tailoring silver nanodots for intracellular staining

• Type of Material: Article
• Authors: Sungmoon Choi, Georgia Institute of Technology; Junhua Yu, Georgia Institute of Technology; Sandeep A. Patel, Georgia Institute of Technology; Yih-Ling Tzeng, Emory University; Robert M. Dickson, Georgia Institute of Technology
• Language: English
• Date: 2011-01
• Publisher: Royal Society of Chemistry
• Publication Version: Author Accepted Manuscript (After Peer Review)
• Copyright Statement: © The Royal Society of Chemistry and Owner Societies 2011
• Final Published Version (URL): http://pubs.rsc.org/en/Content/ArticleLanding/2011/PP/c0pp00263a
• Title of Journal or Parent Work: Photochemical & Photobiological Sciences Photochemical and Photobiological Sciences
• ISSN: 1474-905X
• Volume: 10
• Issue: 1
• Start Page: 109
• End Page: 115
• Grant/Funding Information: Y.-L. T. acknowledges support from NIH R01-AI061031.; BPAE cells were a gift from Prof. J. Karlsson (Drexel University), and NIH 3T3 cells were a gift from Prof. D. Doyle (Georgia Institute of Technology).; R. M. D. gratefully acknowledges financial support from NIH R01-GM086195.
• Research Categories: Engineering, Biomedical; Chemistry, Biochemistry

Abstract

Through tailored oligonucleotide scaffolds, Ag nanocluster syntheses have yielded thermally and cell culture stable silver cluster-based emitters. Optimizing ssDNA stability has enabled creation of highly concentrated and spectrally pure nanocluster emitters with strong intracellular emission. Both fixed and live-cell staining become possible, and intracellular delivery is demonstrated both through conjugation to cell penetrating peptides and via microinjection.