Integrated Traditional Chinese Medicine Improves Functional Outcome in Acute Ischemic Stroke: From Clinic to Mechanism Exploration With Gut Microbiota

• Type of Material: Article
• Authors: Qian Guo, Xuanwu Hospital, Capital Medical University; Can Ni, Xuanwu Hospital, Capital Medical University; Linjing Li, Beijing Chaoyang Integrative Medicine Emergency Medical Center; Mo Li, State Key Laboratory for Turbulence & Complex System; Xiaoqing Jiang, State Key Laboratory for Turbulence & Complex System; Li Gao, Xuanwu Hospital, Capital Medical University; Huaiqiu Zhu, Emory University; Juexian Song, Xuanwu Hospital, Capital Medical University
• Language: English
• Date: 2022-02-09
• Publisher: Emory University Libraries
• Publication Version: Final Published Version
• Copyright Statement: © 2022 Guo, Ni, Li, Li, Jiang, Gao, Zhu and Song
• License: Creative Commons Attribution 4.0 International
• Final Published Version (URL): http://dx.doi.org/10.3389/fcimb.2022.827129
• Title of Journal or Parent Work: Frontiers in Cellular and Infection Microbiology
• Volume: 12
• Start Page: 827129
• End Page: 827129
• Grant/Funding Information: This work was supported by the National Key Research and Development Program of China (2021YFC2300300), the Science and Technology Program of Beijing (Z171100001717012), the Key Special Project of Ministry of Science and Technology Research on modernization of Traditional Chinese Medicine (2019YFC1712400), and the National Natural Science Foundation of China (32070667, 31671366).
• Keywords: Humans; gut microbiota; enterotype; acute ischemic stroke; therapeutic efficacy; Adult; Ischemic Stroke; RNA, Ribosomal, 16S; integrated traditional Chinese medicine; Microbiota; Gastrointestinal Microbiome; Medicine, Chinese Traditional
• Research Categories: Engineering, Biomedical

Abstract

As a life-threatening disease, stroke is the leading cause of death and also induces adult disability worldwide. To investigate the efficacy of the integrated traditional Chinese medicine (ITCM) on the therapeutic effects of acute ischemic stroke (AIS) patients, we enrolled 26 patients in the ITCM [Tanhuo decoction (THD) + Western medicine (WM)] group and 23 in the WM group. Thirty healthy people were also included in the healthy control (HC) group. ITCM achieved better functional outcomes than WM, including significant reduction of the phlegm-heat syndrome and neurological impairment, and improvement of ability. These facts were observed in different pretreatment gut enterotypes. In this paper, we collected the stool samples of all participants and analyzed the 16S rRNA sequence data of the gut microbiota. We identified two enterotypes (Type-A and Type-B) of the gut microbial community in AIS samples before treatment. Compared to Type-B, Type-A was characterized by a high proportion of Bacteroides, relatively high diversity, and severe functional damage. In the ITCM treatment group, we observed better clinical efficacy and positive alterations in microbial diversity and beneficial bacterial abundance, and the effect of approaching healthy people’s gut microbiota, regardless of gut enterotypes identified in pretreatment. Furthermore, we detected several gut microbiota as potential therapeutic targets of ITCM treatment by analyzing the correlations between bacterial abundance alterations and functional outcomes, where Dorea with the strongest correlation was known to produce anti-inflammatory metabolite and negatively linked to trimethylamine-N-oxide (TMAO), a biomarker of AIS. This study analyzed clinical and gut microbial data and revealed the possibility of a broad application independent of the enterotypes, as well as the therapeutic targets of the ITCM in treating AIS patients with phlegm-heat syndrome.