Proteomic analysis of microbial induced redox-dependent intestinal signaling

• Type of Material: Article
• Authors: Jason Matthews, Emory University; April R. Reedy, Emory University; Huixia Wu, Emory University; Benjamin H. Hinrichs, Emory University; Trevor M. Darby, Emory University; Caroline Addis, Emory University; Brian Robinson, Emory University; Young-Mi Go, Emory University; Dean Jones, Emory University; Rheinallt Jones, Emory University; Andrew Neish, Emory University
• Language: English
• Date: 2019-01-01
• Publisher: Elsevier: Creative Commons Licenses
• Publication Version: Final Published Version
• Copyright Statement: © 2018 The Authors
• License: Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International
• Final Published Version (URL): http://dx.doi.org/10.1016/j.redox.2018.11.011
• Title of Journal or Parent Work: Redox Biology
• ISSN: 2213-2317
• Volume: 20
• Start Page: 526
• End Page: 532
• Grant/Funding Information: ARR is funded by T32DK007771-06 training grant.; TMD is funded by a Crohn’s and Colitis Foundation of America (CCFA).; ASN is supported, in part, by NIH R01DK071604 and RO1AI064462.; JDM is funded by a Crohn’s and Colitis Foundation of America (CCFA, CDA#451678).; RMJ is supported in part by NIH Grant RO1 CA179424 and R01DK098391.; BRR is funded by T32DK108735-03 training grant.
• Keywords: Science & Technology; PROTEINS; Biochemistry & Molecular Biology; EPITHELIAL RESTITUTION; Life Sciences & Biomedicine; FORMYL PEPTIDE RECEPTOR; CELLS; ENDOSOMES; NADPH OXIDASE; GUT MICROBIOTA; ACTIVATION; REACTIVE OXYGEN; INJURY
• Research Categories: Health Sciences, Pathology; Biology, Molecular

Abstract

Intestinal homeostasis is regulated in-part by reactive oxygen species (ROS) that are generated in the colonic mucosa following contact with certain lactobacilli. Mechanistically, ROS can modulate protein function through the oxidation of cysteine residues within proteins. Recent advances in cysteine labeling by the Isotope Coded Affinity Tags (ICATs) technique has facilitated the identification of cysteine thiol modifications in response to stimuli. Here, we used ICATs to map the redox protein network oxidized upon initial contact of the colonic mucosa with Lactobacillus rhamnosus GG (LGG). We detected significant LGG-specific redox changes in over 450 proteins, many of which are implicated to function in cellular processes such as endosomal trafficking, epithelial cell junctions, barrier integrity, and cytoskeleton maintenance and formation. We particularly noted the LGG-specific oxidation of Rac1, which is a pleiotropic regulator of many cellular processes. Together, these data reveal new insights into lactobacilli-induced and redox-dependent networks involved in intestinal homeostasis.