Differences in the Constitutive and SIV Infection Induced Expression of Siglecs by Hematopoietic Cells from Non-Human Primates

• Type of Material: Article
• Authors: Jiraporn Jaroenpool, Emory University; Kenneth A. Rogers, Emory University; Kovit Pattanapanyasat, Mahidol University; Francois Villinger, Emory University; Nattawat Onlamoon, Emory University; Paul R. Crocker, University of Dundee; Aftab A Ansari, Emory University
• Language: English
• Date: 2007
• Publisher: Elsevier
• Publication Version: Author Accepted Manuscript (After Peer Review)
• Copyright Statement: © 2008 Elsevier Inc. All rights reserved.
• License: Creative Commons Attribution-NonCommerical-NoDerivs 3.0 Unported
• Final Published Version (URL): http://dx.doi.org/10.1016/j.cellimm.2008.01.009
• Title of Journal or Parent Work: Cellular Immunology
• ISSN: 0008-8749
• Volume: 250
• Issue: 1-2
• Start Page: 91
• End Page: 104
• Grant/Funding Information: Supported by NIH RO1 AI 27057 and by the Thailand Research Fund; The authors are grateful to the Division of Research Resources of the NIH for their support of the Primate Center by the base grant RR-00165.
• Keywords: hematopoietic cell lineages; monocytes; Siglec; non-human primate; SIV infection
• Research Categories: Health Sciences, Pathology

Abstract

The expression of the Siglec family of molecules by hematopoietic cells from uninfected and SIV infected disease susceptible rhesus macaques (RM) and SIV infected disease resistant sooty mangabeys (SM) and for comparison humans was carried out. The predominant cell lineage in all 3 species expressing Siglec’s was monocytes. The major finding by both a cross sectional and a prospective SIV infection study showed that whereas monocytes from RM show marked increase in each Siglec constitutively expressed, monocytes from SM showed marked decreases in Siglec-1 expression. While monocytes from all 3 species constitutively expressed Siglec-3, human monocytes in addition expressed Siglec-5 and 9 and to a lower density 7, monocytes from RM expressed Siglec-7 and those from SM expressed Siglec-1. Monocytes from all 3 species however expressed mRNA for Siglec’s-1, 5, 7 and 9. The reasons for the failure to detect these molecules at the protein level and the mechanisms for such distinct effects of SIV infection on Siglec expression are discussed.