Estimating absolute indoor density of Aedes aegypti using removal sampling

• Type of Material: Article
• Authors: Edgar Koyoc-Cardena, Universidad Autónoma de Yucatán; Anuar Medina-Barreiro, Universidad Autónoma de Yucatán; Azael Cohuo-Rodriguez, Universidad Autónoma de Yucatán; Norma Pavia-Ruz, Universidad Autónoma de Yucatán; Audrey Lenhart, Emory University; Guadalupe Ayora-Talavera, Universidad Autónoma de Yucatán; Mike Dunbar, Emory University; Pablo Manrique-Saide, Emory University; Gonzalo Vazquez Prokopec, Emory University
• Language: English
• Date: 2019-05-21
• Publisher: BioMed Central
• Publication Version: Final Published Version
• Copyright Statement: © The Author(s) 2019
• License: Creative Commons Attribution 4.0 International
• Final Published Version (URL): http://dx.doi.org/10.1186/s13071-019-3503-y
• Title of Journal or Parent Work: Parasites & Vectors
• Volume: 12
• Grant/Funding Information: Research funding was provided by the Bureau for Global Health, U.S. Agency for International Development (USAID:AID‐OAA‐F‐16‐00094; PI, Devine G.), and by an Interagency Agreement between USAID and the US Centers for Disease Control and Prevention (CDC: OADS BAA 2016-N-17844; PI, Vazquez-Prokopec G.M.), by the Canadian Institutes of Health Research (CIHR) and IDRC (Preventing Zika disease with novel vector control approaches, Project 108412) and by Fondo Mixto CONACyT (Mexico)-Gobierno del Estado de Yucatan (Project YUC-2017-03-01-556).
• Keywords: entomology; sampling; population abundance
• Research Categories: Biology, Entomology

Abstract

Quantification of adult Aedes aegypti abundance indoors has relied on estimates of relative density (e.g. number of adults per unit of sampling or time), most commonly using traps or timed collections using aspirators. The lack of estimates of the sensitivity of collections and lack of a numerical association between relative and the absolute density of adult Ae. aegypti represent a significant gap in vector surveillance. Here, we describe the use of sequential removal sampling to estimate absolute numbers of indoor resting Ae. aegypti and to calculate calibration coefficients for timed Prokopack aspirator collections in the city of Merida, Yucatan State, Mexico. The study was performed in 200 houses that were selected based on recent occurrence of Aedes-borne viral illness in residents. Removal sampling occurred in 10-minute sampling rounds performed sequentially until no Ae. aegypti adult was collected for 3 hours or over 2 consecutive 10-minute periods.