HPLC-MS/MS Method for the Measurement of Insecticide Degradates in Baby Food

• Type of Material: Article
• Authors: Samantha Radford, Emory University; Parinya Panuwet, Emory University; Ronald E. Hunter.Jr., Emory University; Dana Barr, Emory University; P Ryan, Emory University
• Language: English
• Date: 2014-07-23
• Publisher: American Chemical Society
• Publication Version: Final Published Version
• Copyright Statement: © 2014 American Chemical Society. ACS AuthorChoice - This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
• Final Published Version (URL): http://dx.doi.org/10.1021/jf500779a
• Title of Journal or Parent Work: Journal of Agricultural and Food Chemistry
• ISSN: 0021-8561
• Volume: 62
• Issue: 29
• Start Page: 7085
• End Page: 7091
• Grant/Funding Information: S.A.R. was supported by Graduate and Postdoctoral Training in Toxicology Grant T32 ES012870.; This work was partially supported by NIH Grant 5RC1ES01829902 under the American Recovery and Reinvestment Act of 2009 and the National Children’s Study under Contract HHSN267200700007C.
• Keywords: degradation; dietary intake; method development; insecticide; solid phase extraction
• Research Categories: Chemistry, General; Health Sciences, Public Health

Abstract

A solid phase extraction method was developed to isolate four insecticide degradates from baby food that were measured subsequently using high-performance liquid chromatography–tandem mass spectrometry. The degradates [parent insecticide] measured were malathion dicarboxylic acid [malathion], 3,5,6-trichloro-2-pyridinol [chlorpyrifos, chlorpyrifos methyl] (TCPy), cis/trans-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropane-1-carboxylic acid [permethrin, cypermethrin, cyfluthrin], and 3-phenoxybenzoic acid [general pyrethroid]. All degradates produced recoveries between 80 and 120% except TCPy in fruit (122% recovery), and all relative standard deviations were <16%. Use of this method demonstrated that insecticide degradates were found in baby foods frequently purchased in the United States, supporting the need for this method. These data will assist in differentiating whether biomarker levels of insecticide metabolites are the result of exposures to the toxic insecticide or its preformed degradate.