Proteomics Links Ubiquitin Chain Topology Change to Transcription Factor Activation

• Type of Material: Article
• Authors: Yanchang Li, Beijing Proteome Research Center; Eric Dammer, Emory University; Yuan Gao, Emory University; Qiuyan Lan, Wuhan University; Mark A. Villamil, University of California, Irvine; Duc M. Duong, Beijing Proteome Research Center; Chengpu Zhang, Beijing Proteome Research Center; Lingyan Ping, Emory University; Linda Lauinger, University of California, Irvine; Karin Flick, University of California, Irvine; Zhongwei Xu, Beijing Proteome Research Center; Wei Wei, Beijing Proteome Research Center; Xiaohua Xing, Beijing Proteome Research Center; Lei Chang, Beijing Proteome Research Center; Jianping Jin, Zhejiang University; Xuechuan Hong, Wuhan University; Yunping Zhu, Beijing Proteome Research Center; Junzhu Wu, Wuhan University; Zixin Deng, Wuhan University; Fuchu He, Beijing Proteome Research Center; Peter Kaiser, University of California, Irvine; Ping Xu, Beijing Proteome Research Center
• Language: English
• Date: 2019-10-03
• Publisher: CELL PRESS
• Publication Version: Author Accepted Manuscript (After Peer Review)
• Copyright Statement: 2019
• License: Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International
• Final Published Version (URL): http://dx.doi.org/10.1016/j.molcel.2019.07.001
• Title of Journal or Parent Work: MOLECULAR CELL
• Volume: 76
• Issue: 1
• Start Page: 126
• End Page: +
• Grant/Funding Information: This work was funded by the MOST (2017YFC0906600, 2017YFA0505002, 2017YFA0505100 & 2016YFA0501300), the National Natural Science Foundation of China (91839302, 31670834, 31700723 & 31870824), Innovation Foundation of Medicine (16CXZ027, BWS17J032 & BWS14J052), National Megaprojects for Key Infectious Diseases (2018ZX10302302001), the Foundation of State Key Lab of Proteomics (SKLP-Y201501 & SKLP-K201705), Guangzhou science and technology innovation & development project (201802020016), the Hitachi-Nomura Award to L. L., and the National Institute of Health (R01 GM-066164 to P.K. and T32 CA09054 to MA.V.).
• Keywords: CHROMATOGRAPHY; QUANTIFICATION; PEPTIDES; MET4; LIGASE; GLOBAL ANALYSIS; PROTEOLYSIS; DEGRADATION; Science & Technology; Life Sciences & Biomedicine; BINDING DOMAINS; QUANTITATIVE PROTEOMICS; Biochemistry & Molecular Biology; Cell Biology
• Research Categories: Chemistry, Biochemistry; Biology, Cell; Biology, Molecular

Abstract

Li et al. discovered that a chain topology change from K48 to K11 linkages on the transcription factor Met4 reduces competition between the K48 chain and the basal transcription complex for binding to the Met4 tandem UBD. These findings provided insight into the role of ubiquitin chain topologies in ubiquitin signaling.