Flow-dependent regulation of genome-wide mRNA and microRNA expression in endothelial cells in vivo.

• Type of Material: Article
• Authors: Sandeep Kumar, Emory University; Chan Woo Kim, Georgia Institute of Technology; Dong Ju Son, Kyungpook National University; Chih Wen Ni, Khalifa University of Science, Technology and Research; Hanjoong Jo, Emory University
• Language: English
• Date: 2014
• Publisher: Nature Publishing Group: Open Access Journals
• Publication Version: Final Published Version
• Copyright Statement: © 2014, Macmillan Publishers Limited
• License: Creative Commons Attribution 4.0 International
• Final Published Version (URL): http://dx.doi.org/10.1038/sdata.2014.39
• Title of Journal or Parent Work: Scientific Data
• ISSN: 2052-4463
• Volume: 1
• Start Page: 140039
• End Page: 140039
• Grant/Funding Information: This work was also supported by the National Heart Lung and Blood Institute of the NIH as a Program of Excellence in Nanotechnology award HHSN268201000043C to HJ.; This work was supported by funding from NIH grants HL095070, HL114772, HL113451 to HJ.
• Keywords: endothelial cells; carotids; microRNA; miR-712; mRNA
• Research Categories: Health Sciences, General; Engineering, Biomedical

Abstract

Atherosclerosis preferentially occurs in arterial regions exposed to disturbed blood flow (d-flow), in part, due to alterations in gene expression in the endothelium. While numerous in vitro studies have shown how anti-atherogenic flow and pro-atherogenic flow differently regulate gene expression of cultured endothelial cells, similar in vivo studies have been scarce. Recently, we developed a mouse model of atherosclerosis that rapidly develops robust atherosclerosis by partially ligating the left carotid artery (LCA) branches, while using the contralateral right carotid (RCA) as control. We also developed a novel method to collect endothelial-enriched RNAs from the carotids of these animals, which enabled us to perform genome-wide expression analyses of mRNAs and miRNAs in the arterial endothelium exposed to either d-flow or s-flow. These microarray results were used to identify novel mechanosensitive genes such as DNA methyltransferase-1 and miR-712 that play key roles in atherosclerosis. Here, we report these endothelial mRNA and miRNA expression profiles with in-depth information on experimental procedures along with an example of usage of these data.